Octylphenol and nonylphenol affect decidualization of human endometrial stromal cells

Duck Sung Ko; Dong Ryul Lee; Haengseok Song; Jong-Hoon Kim; Chun Kyu Lim

doi:10.1016/j.reprotox.2019.06.003

Octylphenol and nonylphenol affect decidualization of human endometrial stromal cells

Duck Sung Ko, Dong Ryul Lee, Haengseok Song, Jong-Hoon Kim, Chun Kyu Lim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

This study investigated the effect of octylphenol (OP) and nonylphenol (NP) on decidualization of endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells were cultured for 12 days and decidualization was induced in vitro. The cells were exposed to OP (5 or 10 μM) or NP (5 μM) during in vitro decidualization. The expression levels of decidualization markers were analyzed using qRT-PCR. The amount of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1) was measured in medium using ELISA. The expression levels of PRL and IGFBP1 were significantly decreased by treatment with OP or NP. The amounts of PRL and IGFBP1 were also significantly decreased by treatment with OP or NP. The patterns of PRL and IGFBP1 secretion into medium were consistent with their expression patterns. The findings indicate that OP or NP may disrupt the decidualization of endometrial stromal cells and eventually affect the reproductive health of women.

Original language	English
Pages (from-to)	13-20
Number of pages	8
Journal	Reproductive Toxicology
Volume	89
DOIs	https://doi.org/10.1016/j.reprotox.2019.06.003
Publication status	Published - 2019 Oct 1

Keywords

Decidualization
Endocrine disrupting chemical
Endometrial stromal cell
Nonylphenol
Octylphenol

ASJC Scopus subject areas

Toxicology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.reprotox.2019.06.003

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title = "Octylphenol and nonylphenol affect decidualization of human endometrial stromal cells",

abstract = "This study investigated the effect of octylphenol (OP) and nonylphenol (NP) on decidualization of endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells were cultured for 12 days and decidualization was induced in vitro. The cells were exposed to OP (5 or 10 μM) or NP (5 μM) during in vitro decidualization. The expression levels of decidualization markers were analyzed using qRT-PCR. The amount of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1) was measured in medium using ELISA. The expression levels of PRL and IGFBP1 were significantly decreased by treatment with OP or NP. The amounts of PRL and IGFBP1 were also significantly decreased by treatment with OP or NP. The patterns of PRL and IGFBP1 secretion into medium were consistent with their expression patterns. The findings indicate that OP or NP may disrupt the decidualization of endometrial stromal cells and eventually affect the reproductive health of women.",

keywords = "Decidualization, Endocrine disrupting chemical, Endometrial stromal cell, Nonylphenol, Octylphenol",

author = "Ko, {Duck Sung} and Lee, {Dong Ryul} and Haengseok Song and Jong-Hoon Kim and Lim, {Chun Kyu}",

year = "2019",

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doi = "10.1016/j.reprotox.2019.06.003",

language = "English",

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AU - Ko, Duck Sung

AU - Lee, Dong Ryul

AU - Song, Haengseok

AU - Kim, Jong-Hoon

AU - Lim, Chun Kyu

PY - 2019/10/1

Y1 - 2019/10/1

N2 - This study investigated the effect of octylphenol (OP) and nonylphenol (NP) on decidualization of endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells were cultured for 12 days and decidualization was induced in vitro. The cells were exposed to OP (5 or 10 μM) or NP (5 μM) during in vitro decidualization. The expression levels of decidualization markers were analyzed using qRT-PCR. The amount of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1) was measured in medium using ELISA. The expression levels of PRL and IGFBP1 were significantly decreased by treatment with OP or NP. The amounts of PRL and IGFBP1 were also significantly decreased by treatment with OP or NP. The patterns of PRL and IGFBP1 secretion into medium were consistent with their expression patterns. The findings indicate that OP or NP may disrupt the decidualization of endometrial stromal cells and eventually affect the reproductive health of women.

AB - This study investigated the effect of octylphenol (OP) and nonylphenol (NP) on decidualization of endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells were cultured for 12 days and decidualization was induced in vitro. The cells were exposed to OP (5 or 10 μM) or NP (5 μM) during in vitro decidualization. The expression levels of decidualization markers were analyzed using qRT-PCR. The amount of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1) was measured in medium using ELISA. The expression levels of PRL and IGFBP1 were significantly decreased by treatment with OP or NP. The amounts of PRL and IGFBP1 were also significantly decreased by treatment with OP or NP. The patterns of PRL and IGFBP1 secretion into medium were consistent with their expression patterns. The findings indicate that OP or NP may disrupt the decidualization of endometrial stromal cells and eventually affect the reproductive health of women.

KW - Decidualization

KW - Endocrine disrupting chemical

KW - Endometrial stromal cell

KW - Nonylphenol

KW - Octylphenol

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Octylphenol and nonylphenol affect decidualization of human endometrial stromal cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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