Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility

Yong Guk Kang; Raymart Jay E. Canoy; Yongjun Jang; Ana Rita M.P. Santos; Inwoo Son; Beop Min Kim; Yongdoo Park

doi:10.1364/BOE.478264

Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility

Yong Guk Kang, Raymart Jay E. Canoy, Yongjun Jang, Ana Rita M.P. Santos, Inwoo Son, Beop Min Kim, Yongdoo Park

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases.

Original language	English
Pages (from-to)	577-592
Number of pages	16
Journal	Biomedical Optics Express
Volume	14
Issue number	2
DOIs	https://doi.org/10.1364/BOE.478264
Publication status	Published - 2023 Feb 1

Bibliographical note

Publisher Copyright:
© 2023 Optica Publishing Group.

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1364/BOE.478264

Cite this

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abstract = "Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases.",

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AU - Son, Inwoo

AU - Kim, Beop Min

AU - Park, Yongdoo

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Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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