Optical diffraction tomography for high resolution live cell imaging

Yongjin Sung; Wonshik Choi; Christopher Fang-Yen; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1364/ntm.2009.ntua2

Optical diffraction tomography for high resolution live cell imaging

Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

College of Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

Original language	English
Title of host publication	Novel Techniques in Microscopy, NTM 2009
Publisher	Optical Society of America (OSA)
ISBN (Print)	9781557528711
DOIs	https://doi.org/10.1364/ntm.2009.ntua2
Publication status	Published - 2009
Event	Novel Techniques in Microscopy, NTM 2009 - Vancouver, Canada Duration: 2009 Apr 26 → 2009 Apr 30

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Other

Other	Novel Techniques in Microscopy, NTM 2009
Country/Territory	Canada
City	Vancouver
Period	09/4/26 → 09/4/30

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics

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10.1364/ntm.2009.ntua2

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@inproceedings{1d0c721f45ca4d2f95120365ef94814c,

title = "Optical diffraction tomography for high resolution live cell imaging",

abstract = "We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.",

author = "Yongjin Sung and Wonshik Choi and Christopher Fang-Yen and Kamran Badizadegan and Dasari, {Ramachandra R.} and Feld, {Michael S.}",

year = "2009",

doi = "10.1364/ntm.2009.ntua2",

language = "English",

isbn = "9781557528711",

series = "Optics InfoBase Conference Papers",

publisher = "Optical Society of America (OSA)",

booktitle = "Novel Techniques in Microscopy, NTM 2009",

note = "Novel Techniques in Microscopy, NTM 2009 ; Conference date: 26-04-2009 Through 30-04-2009",

}

TY - GEN

T1 - Optical diffraction tomography for high resolution live cell imaging

AU - Sung, Yongjin

AU - Choi, Wonshik

AU - Fang-Yen, Christopher

AU - Badizadegan, Kamran

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

PY - 2009

Y1 - 2009

N2 - We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

AB - We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

UR - http://www.scopus.com/inward/record.url?scp=85088186704&partnerID=8YFLogxK

U2 - 10.1364/ntm.2009.ntua2

DO - 10.1364/ntm.2009.ntua2

M3 - Conference contribution

AN - SCOPUS:85088186704

SN - 9781557528711

T3 - Optics InfoBase Conference Papers

BT - Novel Techniques in Microscopy, NTM 2009

PB - Optical Society of America (OSA)

T2 - Novel Techniques in Microscopy, NTM 2009

Y2 - 26 April 2009 through 30 April 2009

ER -

Optical diffraction tomography for high resolution live cell imaging

Abstract

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