Abstract
Pseudomonas sp. PA09 was isolated from farm soil and used for the optical resolution of D-pipecolic acid from DL-pipecolic acid. The strain PA09 consumed L-pipecolic acid preferentially as the sole carbon and energy source, thus accumulating D-pipecolic acid in the culture broth. Optimization to improve the enantiomeric excess and yield was performed. The time course experiment showed that the strain PA09 consumed L-pipecolic acid almost to completion after 25 h of cultivation, and the enantiomeric excess and the yield (% of residual D-pipecolic acid) were 99.8 and 96.0%, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 217-221 |
| Number of pages | 5 |
| Journal | Journal of microbiology and biotechnology |
| Volume | 11 |
| Issue number | 2 |
| Publication status | Published - 2001 Apr |
Keywords
- D-pipecolic acid
- Fermentation
- Optical resolution
- Pseudomonas sp.
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology