Abstract
Response surface methodology (RSM) was performed to maximize the mercury (Hg) reduction from yellowfin tuna (Thunnus albacare) by products protein isolates (YBPI). The optimal condition of Hg reduction (89.3%) was 10.5 mM CaCl2 and a Water:YB of 12.9:1, while other variables were fixed at 5 mM citric acid, 60 min incubation, pH 11 and 8,000 x g for 15 min of centrifugation. At these conditions, the significant protein recovery (80.1%) was obtained. Hydrolysates sequentially hydrolyzed with G6 followed by GN exhibited the highest angiotensin I-converting enzyme (ACE) inhibitory activity than other enzyme preparations. Fractionated yellowfin tuna by products protein hydrolysate (YBPH) increased in ACE-inhibitory activity when peptide size decreased. In-vitro gastrointestinal (GI) digestion significantly increased bioactive property. ACEinhibitory activity of YBPH with and without simulated GI digestion significantly increased after incubating against ACE, demonstrating pro-drug type peptides.
Original language | English |
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Pages (from-to) | 439-447 |
Number of pages | 9 |
Journal | Songklanakarin Journal of Science and Technology |
Volume | 38 |
Issue number | 4 |
Publication status | Published - 2016 |
Bibliographical note
Publisher Copyright:© 2009 RDO All Rights Reserved.
Keywords
- Commercial enzymes
- Gastrointestinal digestion
- Pro-drug type peptides
- Response surface methodology
ASJC Scopus subject areas
- General