Optimization for biodegradation of 2,4,6-trinitrotoluene (TNT) by Pseudomonas putida

Chulhwan Park, Tak Hyun Kim, Sangyong Kim, Seung Wook Kim, Jinwon Lee, Sun Hwan Kim

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


In this study, a strain of Pseudomonas putida KP-T202, isolated from the soil in a contaminated site, degraded 2,4,6-trinitrotoluene (TNT). In order to make this biodegradation process commercially feasible and reduce biodegradation time, optimal environmental factors are determined. At an initial concentration of 100 mg/l, TNT was totally degraded within 15h under aerobic conditions. The optimal conditions for the biodegradation of TNT were found to be 30°C, pH 7, 1% corn steep liquor (CSL), 0.025% NH4Cl and 0.1% Tween 80; the reaction rate constant was 0.348 h-1. These environmental conditions can be used to improve the efficiency of large-scale reactors for the treatment of TNT-contaminated wastewater and soil. In addition, the intermediates were identified as 2-amino-4,6-dinitrotoluene, 4-amino-2,6-dinitrotoluene, 2,4-dinitrotoluene and 2,6-dinitrotoluene.

Original languageEnglish
Pages (from-to)567-571
Number of pages5
JournalJournal of Bioscience and Bioengineering
Issue number6
Publication statusPublished - 2003

Bibliographical note

Funding Information:
This research was supported by the Korea Ministry of Science and Technology (National Research Laboratory Program). The authors greatly appreciate the financial support.


  • Biodegradation
  • Energetic materials
  • Pseudomonas putida
  • Reaction rate constant
  • TNT

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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