TY - JOUR
T1 - Optimization for biodegradation of 2,4,6-trinitrotoluene (TNT) by Pseudomonas putida
AU - Park, Chulhwan
AU - Kim, Tak Hyun
AU - Kim, Sangyong
AU - Kim, Seung Wook
AU - Lee, Jinwon
AU - Kim, Sun Hwan
N1 - Funding Information:
This research was supported by the Korea Ministry of Science and Technology (National Research Laboratory Program). The authors greatly appreciate the financial support.
PY - 2003
Y1 - 2003
N2 - In this study, a strain of Pseudomonas putida KP-T202, isolated from the soil in a contaminated site, degraded 2,4,6-trinitrotoluene (TNT). In order to make this biodegradation process commercially feasible and reduce biodegradation time, optimal environmental factors are determined. At an initial concentration of 100 mg/l, TNT was totally degraded within 15h under aerobic conditions. The optimal conditions for the biodegradation of TNT were found to be 30°C, pH 7, 1% corn steep liquor (CSL), 0.025% NH4Cl and 0.1% Tween 80; the reaction rate constant was 0.348 h-1. These environmental conditions can be used to improve the efficiency of large-scale reactors for the treatment of TNT-contaminated wastewater and soil. In addition, the intermediates were identified as 2-amino-4,6-dinitrotoluene, 4-amino-2,6-dinitrotoluene, 2,4-dinitrotoluene and 2,6-dinitrotoluene.
AB - In this study, a strain of Pseudomonas putida KP-T202, isolated from the soil in a contaminated site, degraded 2,4,6-trinitrotoluene (TNT). In order to make this biodegradation process commercially feasible and reduce biodegradation time, optimal environmental factors are determined. At an initial concentration of 100 mg/l, TNT was totally degraded within 15h under aerobic conditions. The optimal conditions for the biodegradation of TNT were found to be 30°C, pH 7, 1% corn steep liquor (CSL), 0.025% NH4Cl and 0.1% Tween 80; the reaction rate constant was 0.348 h-1. These environmental conditions can be used to improve the efficiency of large-scale reactors for the treatment of TNT-contaminated wastewater and soil. In addition, the intermediates were identified as 2-amino-4,6-dinitrotoluene, 4-amino-2,6-dinitrotoluene, 2,4-dinitrotoluene and 2,6-dinitrotoluene.
KW - Biodegradation
KW - Energetic materials
KW - Pseudomonas putida
KW - Reaction rate constant
KW - TNT
UR - http://www.scopus.com/inward/record.url?scp=0038344486&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(03)80163-9
DO - 10.1016/S1389-1723(03)80163-9
M3 - Article
C2 - 16233458
AN - SCOPUS:0038344486
SN - 1389-1723
VL - 95
SP - 567
EP - 571
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 6
ER -