Abstract
Production of lincomycin by Streptomyces lincolnensis was studied by varying medium composition and environmental conditions. With the medium composition optimized by statistical experimental design (45 g/L soluble starch, 15 g/L sugar cane molasses, 13.33 g/L peptone water, 6.67 g/L NaNO3 and 4.0 g/L CaCO3), lincomycin production increased by 2 ∼ 3 fold compared to that obtained with un-optimized basal medium. Lincomycin production was further improved by optimizing culture conditions such as agitation speed, impeller type and pH under the optimized medium condition. The highest titer of 350 mg/L lincomycin was achieved from 240 h bioreactor culture. These results demonstrate that fermentation conditions for maximal lincomycin production by Streptomyces lincolnensis were optimized via biotechnological processes.
| Original language | English |
|---|---|
| Pages (from-to) | 1014-1021 |
| Number of pages | 8 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2014 Nov |
| Externally published | Yes |
Bibliographical note
Funding Information:Financial support was provided by the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. The authors are grateful to all the members of the Research Center for Industrial Development of Biofood Materials, Chonbuk National University, for their helpful discussion and intellectual interactions.
Publisher Copyright:
© 2014, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
Keywords
- Streptomyces lincolnensis
- fermentation
- lincomycin
- medium optimization
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering