Abstract
Culture medium and conditions were optimized to improve neo-fructooilgosaccharide (neo-FOS) production by Penicillium citrinum using Response Surface Methodology (RSM). The central composite designs using RSM were employed in this investigation. The quadratic models for neo-FOS production were obtained. Among culture media, optimal concentration of sucrose and yeast extract were found to be 12.65% and 2.90%, respectively, and predicted maximum value of neo-FOS production was 39.3 g/l. For culture conditions, optimal points of inoculum age (78.5 h), initial pH (7.34) and inoculum size (11.36% (v/v)) were determined and predicted maximum value of neo-FOS production was 40.6 g/l. By performing the culture under optimal culture media and conditions, actual neo-FOS production increased to 107%. It was also suggested that RSM can be used as a potential tool for optimization of factors in fermentation and food industry.
| Original language | English |
|---|---|
| Pages (from-to) | 639-644 |
| Number of pages | 6 |
| Journal | European Food Research and Technology |
| Volume | 221 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 Oct |
Bibliographical note
Funding Information:Acknowledgements This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, South Korea.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
Keywords
- Neo-fructooligosaccharide
- Optimization
- Penicillium citrinum
- Response Surface Methodology
- Statistical analysis
ASJC Scopus subject areas
- Biotechnology
- Food Science
- General Chemistry
- Biochemistry
- Industrial and Manufacturing Engineering