Optimization of enzymatic biodiesel synthesis using RSM in high pressure carbon dioxide and its scale up

Myunggu Lee, Dohoon Lee, Jaehoon Cho, Junhac Lee, Sangyong Kim, Seung Wook Kim, Chulhwan Park

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Enzymatic synthesis of biodiesel by the transesterification of canola oil and methanol in high pressure carbon dioxide [HPCO2: near-critical and supercritical carbon dioxide (NcCO2 and ScCO2)] was optimized using response surface methodology (RSM). RSM based on 5-level-5-factor central composite rotatable design (CCRD) was used to evaluate the effects of temperature, pressure, enzyme loading, substrate molar ratio, and time on the conversion to biodiesel by transesterification. Finally, batch reactions for biodiesel synthesis were preformed in a 100 mL and 7 L high-pressure stirred batch reactors.

    Original languageEnglish
    Pages (from-to)775-780
    Number of pages6
    JournalBioprocess and Biosystems Engineering
    Volume36
    Issue number6
    DOIs
    Publication statusPublished - 2013 Jun

    Bibliographical note

    Funding Information:
    Acknowledgments This work was funded by the Industrial Strategic Technology Development Program of the Ministry of Knowledge

    Funding Information:
    Economy (MKE), Korea and by the Basic Research Program of the Korea Institute of Industrial Technology (KITECH). This research was also supported by the Knowledge-based Environmental Service Human Resource Development Project of Korea Ministry of Environment (MOE) and the Research Grant from Kwangwoon University in 2013. The 7 L high-pressure stirred batch reactor was provided by Hanil Corporation, Korea.

    Keywords

    • High pressure carbon dioxide
    • Lipase
    • Optimization
    • Response surface methodology
    • Transesterification

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    Fingerprint

    Dive into the research topics of 'Optimization of enzymatic biodiesel synthesis using RSM in high pressure carbon dioxide and its scale up'. Together they form a unique fingerprint.

    Cite this