Abstract
Enzymatic synthesis of biodiesel by the transesterification of canola oil and methanol in high pressure carbon dioxide [HPCO2: near-critical and supercritical carbon dioxide (NcCO2 and ScCO2)] was optimized using response surface methodology (RSM). RSM based on 5-level-5-factor central composite rotatable design (CCRD) was used to evaluate the effects of temperature, pressure, enzyme loading, substrate molar ratio, and time on the conversion to biodiesel by transesterification. Finally, batch reactions for biodiesel synthesis were preformed in a 100 mL and 7 L high-pressure stirred batch reactors.
Original language | English |
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Pages (from-to) | 775-780 |
Number of pages | 6 |
Journal | Bioprocess and Biosystems Engineering |
Volume | 36 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2013 Jun |
Bibliographical note
Funding Information:Acknowledgments This work was funded by the Industrial Strategic Technology Development Program of the Ministry of Knowledge
Funding Information:
Economy (MKE), Korea and by the Basic Research Program of the Korea Institute of Industrial Technology (KITECH). This research was also supported by the Knowledge-based Environmental Service Human Resource Development Project of Korea Ministry of Environment (MOE) and the Research Grant from Kwangwoon University in 2013. The 7 L high-pressure stirred batch reactor was provided by Hanil Corporation, Korea.
Keywords
- High pressure carbon dioxide
- Lipase
- Optimization
- Response surface methodology
- Transesterification
ASJC Scopus subject areas
- Biotechnology
- Bioengineering