Optimization of hydrogen production by hyperthermophilic eubacteria, Thermotoga maritima and Thermotoga neapolitana in batch fermentation

Tam Anh D. Nguyen; Jun Pyo Kim; Mi Sun Kim; You Kwan Oh; Sang Jun Sim

doi:10.1016/j.ijhydene.2007.09.033

Optimization of hydrogen production by hyperthermophilic eubacteria, Thermotoga maritima and Thermotoga neapolitana in batch fermentation

Tam Anh D. Nguyen, Jun Pyo Kim, Mi Sun Kim, You Kwan Oh, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

The batch fermentations of two hyperthermophilic eubacteria Thermotoga maritima strain DSM 3109 and Thermotoga neapolitana strain DSM 4359 were carried out to optimize the hydrogen production. The simple and economical culture medium using cheap salts with strong buffering capacity was designed based on T. maritima basal medium (TMB). Both strains cultivated under strictly anaerobic conditions showed the best growth at temperature of 75-80 °C and pH of 6.5-7.0. The maximum cell growth of 3.14 g DCW/L and hydrogen production of 342 mL H₂ gas/L were obtained, respectively, in the modified TB medium containing 7.5 g/L of glucose and 4 g/L of yeast extract. Hydrogen accumulation in the headspace was more than 30% of the gaseous phase. Cells were also cultivated in cellulose-containing medium to test the feasibility of hydrogen production.

Original language	English
Pages (from-to)	1483-1488
Number of pages	6
Journal	International Journal of Hydrogen Energy
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/j.ijhydene.2007.09.033
Publication status	Published - 2008 Mar
Externally published	Yes

Keywords

Hydrogen production
Hyperthermophilic eubacteria
Thermotoga

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijhydene.2007.09.033

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title = "Optimization of hydrogen production by hyperthermophilic eubacteria, Thermotoga maritima and Thermotoga neapolitana in batch fermentation",

abstract = "The batch fermentations of two hyperthermophilic eubacteria Thermotoga maritima strain DSM 3109 and Thermotoga neapolitana strain DSM 4359 were carried out to optimize the hydrogen production. The simple and economical culture medium using cheap salts with strong buffering capacity was designed based on T. maritima basal medium (TMB). Both strains cultivated under strictly anaerobic conditions showed the best growth at temperature of 75-80 °C and pH of 6.5-7.0. The maximum cell growth of 3.14 g DCW/L and hydrogen production of 342 mL H2 gas/L were obtained, respectively, in the modified TB medium containing 7.5 g/L of glucose and 4 g/L of yeast extract. Hydrogen accumulation in the headspace was more than 30% of the gaseous phase. Cells were also cultivated in cellulose-containing medium to test the feasibility of hydrogen production.",

keywords = "Hydrogen production, Hyperthermophilic eubacteria, Thermotoga",

author = "Nguyen, {Tam Anh D.} and {Pyo Kim}, Jun and {Sun Kim}, Mi and {Kwan Oh}, You and Sim, {Sang Jun}",

note = "Funding Information: This research was performed for the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2008",

month = mar,

doi = "10.1016/j.ijhydene.2007.09.033",

language = "English",

volume = "33",

pages = "1483--1488",

journal = "International Journal of Hydrogen Energy",

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AU - Nguyen, Tam Anh D.

AU - Pyo Kim, Jun

AU - Sun Kim, Mi

AU - Kwan Oh, You

AU - Sim, Sang Jun

N1 - Funding Information: This research was performed for the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2008/3

Y1 - 2008/3

N2 - The batch fermentations of two hyperthermophilic eubacteria Thermotoga maritima strain DSM 3109 and Thermotoga neapolitana strain DSM 4359 were carried out to optimize the hydrogen production. The simple and economical culture medium using cheap salts with strong buffering capacity was designed based on T. maritima basal medium (TMB). Both strains cultivated under strictly anaerobic conditions showed the best growth at temperature of 75-80 °C and pH of 6.5-7.0. The maximum cell growth of 3.14 g DCW/L and hydrogen production of 342 mL H2 gas/L were obtained, respectively, in the modified TB medium containing 7.5 g/L of glucose and 4 g/L of yeast extract. Hydrogen accumulation in the headspace was more than 30% of the gaseous phase. Cells were also cultivated in cellulose-containing medium to test the feasibility of hydrogen production.

AB - The batch fermentations of two hyperthermophilic eubacteria Thermotoga maritima strain DSM 3109 and Thermotoga neapolitana strain DSM 4359 were carried out to optimize the hydrogen production. The simple and economical culture medium using cheap salts with strong buffering capacity was designed based on T. maritima basal medium (TMB). Both strains cultivated under strictly anaerobic conditions showed the best growth at temperature of 75-80 °C and pH of 6.5-7.0. The maximum cell growth of 3.14 g DCW/L and hydrogen production of 342 mL H2 gas/L were obtained, respectively, in the modified TB medium containing 7.5 g/L of glucose and 4 g/L of yeast extract. Hydrogen accumulation in the headspace was more than 30% of the gaseous phase. Cells were also cultivated in cellulose-containing medium to test the feasibility of hydrogen production.

KW - Hydrogen production

KW - Hyperthermophilic eubacteria

KW - Thermotoga

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JO - International Journal of Hydrogen Energy

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IS - 5

ER -

Optimization of hydrogen production by hyperthermophilic eubacteria, Thermotoga maritima and Thermotoga neapolitana in batch fermentation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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