Optimization of submerged culture conditions for the production of angiotensin converting enzyme inhibitor from Flammulina velutipes

J. M. Kim; K. S. Ra; D. O. Noh; H. J. Suh

doi:10.1038/sj.jim.7000306

Optimization of submerged culture conditions for the production of angiotensin converting enzyme inhibitor from Flammulina velutipes

J. M. Kim, K. S. Ra, D. O. Noh, H. J. Suh

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The angiotensin-converting enzyme (ACE) inhibitory effect was tested in the culture broth from submerged mycelial cultures of 20 basidiomycetes. The ACE inhibitory effect of culture broth from Flammulina velutipes strain 414 was the highest (52.8%), followed by Lentinus edodes strains 2 (44.4%) and 16 (41.3%). Nutritional requirements for the production of ACE inhibitory substance from F. velutipes were studied. Sucrose, ammonium acetate, and glutamic acid were chosen for the maximum production of ACE inhibitory substance. The optimal medium composition was (g/l): sucrose 20, ammonium acetate 5, glutamic acid 2, KH₂PO₄ 3, MgSO₄·7H₂O 0.8, and yeast extract 0.5. Under optimal culture conditions, the ACE inhibitory effect was more than 80%.

Original language	English
Pages (from-to)	292-295
Number of pages	4
Journal	Journal of Industrial Microbiology and Biotechnology
Volume	29
Issue number	5
DOIs	https://doi.org/10.1038/sj.jim.7000306
Publication status	Published - 2002 Nov 1

Keywords

ACE inhibitor
Flammulina velutipes
Optimization
Submerged fermentation

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1038/sj.jim.7000306

Cite this

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abstract = "The angiotensin-converting enzyme (ACE) inhibitory effect was tested in the culture broth from submerged mycelial cultures of 20 basidiomycetes. The ACE inhibitory effect of culture broth from Flammulina velutipes strain 414 was the highest (52.8%), followed by Lentinus edodes strains 2 (44.4%) and 16 (41.3%). Nutritional requirements for the production of ACE inhibitory substance from F. velutipes were studied. Sucrose, ammonium acetate, and glutamic acid were chosen for the maximum production of ACE inhibitory substance. The optimal medium composition was (g/l): sucrose 20, ammonium acetate 5, glutamic acid 2, KH2PO4 3, MgSO4·7H2O 0.8, and yeast extract 0.5. Under optimal culture conditions, the ACE inhibitory effect was more than 80%.",

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AU - Kim, J. M.

AU - Ra, K. S.

AU - Noh, D. O.

AU - Suh, H. J.

PY - 2002/11/1

Y1 - 2002/11/1

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AB - The angiotensin-converting enzyme (ACE) inhibitory effect was tested in the culture broth from submerged mycelial cultures of 20 basidiomycetes. The ACE inhibitory effect of culture broth from Flammulina velutipes strain 414 was the highest (52.8%), followed by Lentinus edodes strains 2 (44.4%) and 16 (41.3%). Nutritional requirements for the production of ACE inhibitory substance from F. velutipes were studied. Sucrose, ammonium acetate, and glutamic acid were chosen for the maximum production of ACE inhibitory substance. The optimal medium composition was (g/l): sucrose 20, ammonium acetate 5, glutamic acid 2, KH2PO4 3, MgSO4·7H2O 0.8, and yeast extract 0.5. Under optimal culture conditions, the ACE inhibitory effect was more than 80%.

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Optimization of submerged culture conditions for the production of angiotensin converting enzyme inhibitor from Flammulina velutipes

Abstract

Keywords

ASJC Scopus subject areas

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