@inproceedings{c444b5c78bdb40458e2ee1a849fbf815,
title = "Optofluidic Surface Enhanced Raman Scattering based detection of single nucleotide polymorphism",
abstract = "Single nucleotide polymorphisms (SNPs) have become key indicators of genetic disease, allelic variation associated with cancer progression, and pharmcogenomics. The ability rapidly screen for SNPs will have a profound impact on a number of applications, most notably personalized medicine. Here we demonstrate a new approach to SNP detection through the application of Surface Enhanced Raman Scattering (SERS) to the Ligase Detection Reaction (LDR). The reaction is implemented in an electrokinetically active microfluidic device that enables physical concentration of the reaction products for enhanced detection sensitivity and quantization. In addition, we present LDR-SERS multiplex SNP detection in the KRas oncogene and the direct correlation of diagnostic peak intensity to SNP concentration in mixed genotype samples.",
keywords = "Ligase detection reaction (LDR), Optofluidic device, Single nucleotide polymorphism (SNP), Surface-enhanced Raman scattering (SERS)",
author = "Huh, {Yun Suk} and Lowe, {Adam J.} and Chung, {Aram J.} and Bernardo Cordovez and Strickland, {Aaron D.} and Batt, {Carl A.} and David Erickson",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "281--283",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}
