TY - GEN
T1 - Optofluidic surface enhanced Raman scattering chip for detection of dengue virus
AU - Yun, Suk Huh
AU - Chung, Aram J.
AU - Cordovez, Bernardo
AU - Erickson, David
PY - 2009
Y1 - 2009
N2 - In this work we describe the development of an optofluidic device for surface enhanced Raman scattering (SERS) based detection of biological pathogens. The chip exploits the use of electro-active microwells which serve to both physically concentrate the Raman enhancers and to reduce the total analysis time through a unique electrokinetically driven on-chip mixing effect. To quantify the concentralion performance of the device we use 44 nm polystyrene particles at low electric field strength (between 1.00-2.00 V) and demonstrate close to 90% concentration saturation within 2.5 s. We demonstrate the mixing capability through the enhanced detection of dengue virus serotype 2 (DENV-2). With DENV-2, we successfully detected the SERS signals with a limit of detection of 30 pM.
AB - In this work we describe the development of an optofluidic device for surface enhanced Raman scattering (SERS) based detection of biological pathogens. The chip exploits the use of electro-active microwells which serve to both physically concentrate the Raman enhancers and to reduce the total analysis time through a unique electrokinetically driven on-chip mixing effect. To quantify the concentralion performance of the device we use 44 nm polystyrene particles at low electric field strength (between 1.00-2.00 V) and demonstrate close to 90% concentration saturation within 2.5 s. We demonstrate the mixing capability through the enhanced detection of dengue virus serotype 2 (DENV-2). With DENV-2, we successfully detected the SERS signals with a limit of detection of 30 pM.
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UR - http://www.scopus.com/inward/citedby.url?scp=70049117811&partnerID=8YFLogxK
U2 - 10.1115/IMECE2008-67243
DO - 10.1115/IMECE2008-67243
M3 - Conference contribution
AN - SCOPUS:70049117811
SN - 9780791848746
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings
SP - 873
EP - 876
BT - 2008 Proceedings of ASME International Mechanical Engineering Congress and Exposition, IMECE 2008
T2 - 2008 ASME International Mechanical Engineering Congress and Exposition, IMECE 2008
Y2 - 31 October 2008 through 6 November 2008
ER -