TY - JOUR
T1 - Opuntia humifusa Partitioned Extracts Inhibit the Growth of U87MG Human Glioblastoma Cells
AU - Hahm, Sahng Wook
AU - Park, Jieun
AU - Son, Yong Suk
N1 - Funding Information:
Acknowledgements This work was supported by a grant (LS 0509) provided by the Agricultural Techniques Center, Rural Development Administration, Republic of Korea.
PY - 2010
Y1 - 2010
N2 - Opuntia humifusa, a member of the Cactaceae family widely distributed in the southern regions of the Korean peninsula, has potential bioactive functions and medicinal benefits. In the present study, we investigated the effect of hexane, ethyl acetate extracts and water partitioned fraction of O. humifusa on proliferation, G1 arrest and apoptosis in U87MG human glioblastoma cells. Glioblastoma cellular proliferation was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the effects of O. humifusa partitioned extracts on cell cycle and apoptosis were analyzed by flow cytometry. Our results revealed that when U87MG cells were treated with hexane extracts and water partitioned fraction of O. humifusa, the number of viable cells decreased in a concentration-dependent manner. In addition, water partitioned fractions of O. humifusa induced G1 arrest and non-apoptotic cell death as well as significant increases in ROS production in U87MG cells. In conclusion, water partitioned fractions of O. humifusa induce G1 arrest and inhibit U87MG human glioblastoma cell proliferation.
AB - Opuntia humifusa, a member of the Cactaceae family widely distributed in the southern regions of the Korean peninsula, has potential bioactive functions and medicinal benefits. In the present study, we investigated the effect of hexane, ethyl acetate extracts and water partitioned fraction of O. humifusa on proliferation, G1 arrest and apoptosis in U87MG human glioblastoma cells. Glioblastoma cellular proliferation was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the effects of O. humifusa partitioned extracts on cell cycle and apoptosis were analyzed by flow cytometry. Our results revealed that when U87MG cells were treated with hexane extracts and water partitioned fraction of O. humifusa, the number of viable cells decreased in a concentration-dependent manner. In addition, water partitioned fractions of O. humifusa induced G1 arrest and non-apoptotic cell death as well as significant increases in ROS production in U87MG cells. In conclusion, water partitioned fractions of O. humifusa induce G1 arrest and inhibit U87MG human glioblastoma cell proliferation.
KW - Antiproliferative activity
KW - G1 arrest
KW - Opuntia humifusa
KW - U87MG
KW - Water partitioned fractions
UR - http://www.scopus.com/inward/record.url?scp=77957134092&partnerID=8YFLogxK
U2 - 10.1007/s11130-010-0188-y
DO - 10.1007/s11130-010-0188-y
M3 - Article
C2 - 20814744
AN - SCOPUS:77957134092
SN - 0921-9668
VL - 65
SP - 247
EP - 252
JO - Plant Foods for Human Nutrition
JF - Plant Foods for Human Nutrition
IS - 3
ER -