Origami-paper-based device for microvesicle/exosome preconcentration and isolation

Hyerin Kim; Kyu Hyoung Lee; Sung Il Han; Dongho Lee; Seok Chung; Dohwan Lee; Jeong Hoon Lee

doi:10.1039/c9lc00796b

Origami-paper-based device for microvesicle/exosome preconcentration and isolation

Hyerin Kim, Kyu Hyoung Lee, Sung Il Han, Dongho Lee, Seok Chung, Dohwan Lee, Jeong Hoon Lee

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Microvesicles and exosomes are promising liquid biopsy biomarkers. However, conventional isolation techniques damage and contaminate the biomarkers. We developed an origami-paper-based device for effective isolation of biomarkers with less damage and in fewer steps. The multi-folded device enables the preconcentration of the microvesicles/exosomes on specific layers (∼5-fold) by the ion concentration polarization technique and they were simply isolated from the rest of the sample by unfolding the device.

Original language	English
Pages (from-to)	3917-3921
Number of pages	5
Journal	Lab on a Chip
Volume	19
Issue number	23
DOIs	https://doi.org/10.1039/c9lc00796b
Publication status	Published - 2019 Dec 7

Bibliographical note

Publisher Copyright:
This journal is © The Royal Society of Chemistry.

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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10.1039/c9lc00796b

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AU - Han, Sung Il

AU - Lee, Dongho

AU - Chung, Seok

AU - Lee, Dohwan

AU - Lee, Jeong Hoon

PY - 2019/12/7

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AB - Microvesicles and exosomes are promising liquid biopsy biomarkers. However, conventional isolation techniques damage and contaminate the biomarkers. We developed an origami-paper-based device for effective isolation of biomarkers with less damage and in fewer steps. The multi-folded device enables the preconcentration of the microvesicles/exosomes on specific layers (∼5-fold) by the ion concentration polarization technique and they were simply isolated from the rest of the sample by unfolding the device.

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Origami-paper-based device for microvesicle/exosome preconcentration and isolation

Abstract

Bibliographical note

ASJC Scopus subject areas

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