Origami paper-based sample preconcentration using sequentially driven ion concentration polarization

Junwoo Lee, Yong Kyoung Yoo, Dohwan Lee, Cheonjung Kim, Kang Hyeon Kim, Seungmin Lee, Seungmin Kwak, Ji Yoon Kang, Hyungsuk Kim, Dae Sung Yoon, Don Hur, Jeong Hoon Lee

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals.

Original languageEnglish
Pages (from-to)867-874
Number of pages8
JournalLab on a Chip
Issue number5
Publication statusPublished - 2021 Mar 7

Bibliographical note

Funding Information:
This work was supported by Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20194010201830). This work was also supported by the Technology development Program (S2848425) by the Ministry of SMEs and Startups (MSS, Korea).

Publisher Copyright:
© The Royal Society of Chemistry 2021.

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering


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