Origami paper-based sample preconcentration using sequentially driven ion concentration polarization

Junwoo Lee; Yong Kyoung Yoo; Dohwan Lee; Cheonjung Kim; Kang Hyeon Kim; Seungmin Lee; Seungmin Kwak; Ji Yoon Kang; Hyungsuk Kim; Dae Sung Yoon; Don Hur; Jeong Hoon Lee

doi:10.1039/d0lc01032d

Origami paper-based sample preconcentration using sequentially driven ion concentration polarization

Junwoo Lee, Yong Kyoung Yoo, Dohwan Lee, Cheonjung Kim, Kang Hyeon Kim, Seungmin Lee, Seungmin Kwak, Ji Yoon Kang, Hyungsuk Kim, Dae Sung Yoon, Don Hur, Jeong Hoon Lee

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals.

Original language	English
Pages (from-to)	867-874
Number of pages	8
Journal	Lab on a Chip
Volume	21
Issue number	5
DOIs	https://doi.org/10.1039/d0lc01032d
Publication status	Published - 2021 Mar 7

Bibliographical note

Funding Information:
This work was supported by Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20194010201830). This work was also supported by the Technology development Program (S2848425) by the Ministry of SMEs and Startups (MSS, Korea).

Publisher Copyright:
© The Royal Society of Chemistry 2021.

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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title = "Origami paper-based sample preconcentration using sequentially driven ion concentration polarization",

abstract = "Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals.",

author = "Junwoo Lee and Yoo, {Yong Kyoung} and Dohwan Lee and Cheonjung Kim and Kim, {Kang Hyeon} and Seungmin Lee and Seungmin Kwak and Kang, {Ji Yoon} and Hyungsuk Kim and Yoon, {Dae Sung} and Don Hur and Lee, {Jeong Hoon}",

note = "Funding Information: This work was supported by Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20194010201830). This work was also supported by the Technology development Program (S2848425) by the Ministry of SMEs and Startups (MSS, Korea). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = mar,

day = "7",

doi = "10.1039/d0lc01032d",

language = "English",

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AU - Lee, Junwoo

AU - Yoo, Yong Kyoung

AU - Lee, Dohwan

AU - Kim, Cheonjung

AU - Kim, Kang Hyeon

AU - Lee, Seungmin

AU - Kwak, Seungmin

AU - Kang, Ji Yoon

AU - Kim, Hyungsuk

AU - Yoon, Dae Sung

AU - Hur, Don

AU - Lee, Jeong Hoon

N1 - Funding Information: This work was supported by Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20194010201830). This work was also supported by the Technology development Program (S2848425) by the Ministry of SMEs and Startups (MSS, Korea). Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/3/7

Y1 - 2021/3/7

N2 - Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals.

AB - Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals.

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Origami paper-based sample preconcentration using sequentially driven ion concentration polarization

Abstract

Bibliographical note

ASJC Scopus subject areas

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