OsFCA transcripts show more complex alternative processing patterns than its Arabidopsis counterparts

Yun Hee Jang; Jeong Hwan Lee; Hyo Young Park; Soon Kap Kim; Bo Young Lee; Mi Chung Suh; Jeong Kook Kim

doi:10.1007/s12374-009-9018-x

OsFCA transcripts show more complex alternative processing patterns than its Arabidopsis counterparts

Yun Hee Jang, Jeong Hwan Lee, Hyo Young Park, Soon Kap Kim, Bo Young Lee, Mi Chung Suh, Jeong Kook Kim

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.

Original language	English
Pages (from-to)	161-166
Number of pages	6
Journal	Journal of Plant Biology
Volume	52
Issue number	2
DOIs	https://doi.org/10.1007/s12374-009-9018-x
Publication status	Published - 2009 Apr

Bibliographical note

Funding Information:
Acknowledgments We are grateful to Dr Kyung Ho Kang for the generous gift of the Korean rice cultivar seeds. This study was supported by a grant from the Plant Signaling Network Research Center, Korea Science and Engineering Foundation Republic of Korea. This study was also supported partly by a grant from the Biogreen 21 program, Rural Development Administration. M.C. Suh was supported by a grant from Agricultural Plant Stress Research Center and Biotechnology Research Institute, Korea Science and Engineering Foundation Republic of Korea. Y.H. Jang, S-K. Kim, and H-Y. Park were supported by a BK21 research fellowship from the Korean Ministry of Education and Human Resources Development. J.H. Lee was supported by the Korea Research Foundation Grant (KRF-2007-359-C00023) funded by the Korea Government (MOEHRD).

Keywords

Alternatively spliced transcripts
Flowering time
OsFCA
Rice

ASJC Scopus subject areas

Plant Science

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10.1007/s12374-009-9018-x

Cite this

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title = "OsFCA transcripts show more complex alternative processing patterns than its Arabidopsis counterparts",

abstract = "The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.",

keywords = "Alternatively spliced transcripts, Flowering time, OsFCA, Rice",

author = "Jang, {Yun Hee} and Lee, {Jeong Hwan} and Park, {Hyo Young} and Kim, {Soon Kap} and Lee, {Bo Young} and Suh, {Mi Chung} and Kim, {Jeong Kook}",

note = "Funding Information: Acknowledgments We are grateful to Dr Kyung Ho Kang for the generous gift of the Korean rice cultivar seeds. This study was supported by a grant from the Plant Signaling Network Research Center, Korea Science and Engineering Foundation Republic of Korea. This study was also supported partly by a grant from the Biogreen 21 program, Rural Development Administration. M.C. Suh was supported by a grant from Agricultural Plant Stress Research Center and Biotechnology Research Institute, Korea Science and Engineering Foundation Republic of Korea. Y.H. Jang, S-K. Kim, and H-Y. Park were supported by a BK21 research fellowship from the Korean Ministry of Education and Human Resources Development. J.H. Lee was supported by the Korea Research Foundation Grant (KRF-2007-359-C00023) funded by the Korea Government (MOEHRD).",

year = "2009",

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doi = "10.1007/s12374-009-9018-x",

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AU - Jang, Yun Hee

AU - Lee, Jeong Hwan

AU - Park, Hyo Young

AU - Kim, Soon Kap

AU - Lee, Bo Young

AU - Suh, Mi Chung

AU - Kim, Jeong Kook

N1 - Funding Information: Acknowledgments We are grateful to Dr Kyung Ho Kang for the generous gift of the Korean rice cultivar seeds. This study was supported by a grant from the Plant Signaling Network Research Center, Korea Science and Engineering Foundation Republic of Korea. This study was also supported partly by a grant from the Biogreen 21 program, Rural Development Administration. M.C. Suh was supported by a grant from Agricultural Plant Stress Research Center and Biotechnology Research Institute, Korea Science and Engineering Foundation Republic of Korea. Y.H. Jang, S-K. Kim, and H-Y. Park were supported by a BK21 research fellowship from the Korean Ministry of Education and Human Resources Development. J.H. Lee was supported by the Korea Research Foundation Grant (KRF-2007-359-C00023) funded by the Korea Government (MOEHRD).

PY - 2009/4

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N2 - The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.

AB - The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.

KW - Alternatively spliced transcripts

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DO - 10.1007/s12374-009-9018-x

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SN - 1226-9239

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JO - Journal of Plant Biology

JF - Journal of Plant Biology

IS - 2

ER -

OsFCA transcripts show more complex alternative processing patterns than its Arabidopsis counterparts

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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