Abstract
The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. Ti substrates were first modified through heparin-dopamine (Hep-DOPA) anchorage. Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. Hep-Ti substrates, with or without LF, were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Sustained release of LF on the Ti substrates was observed over a 28-day period. In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity.
| Original language | English |
|---|---|
| Pages (from-to) | 191-198 |
| Number of pages | 8 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 123 |
| DOIs | |
| Publication status | Published - 2014 Nov 1 |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by a grant from Korea University ( K1300081 ).
Publisher Copyright:
© 2014 Elsevier B.V.
Keywords
- Heparin-dopamine
- Lactoferrin
- Osteoblast
- Osteogenic differentiation
- Titanium
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
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