Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

Jae Yong Lee; Sung Eun Kim; Young Pil Yun; Sung Wook Choi; Daniel I. Jeon; Hak Jun Kim; Kyeongsoon Park; Hae Ryong Song

doi:10.1016/j.jiec.2017.03.057

Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

Jae Yong Lee, Sung Eun Kim, Young Pil Yun, Sung Wook Choi, Daniel I. Jeon, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

In this study, we evaluated in vitro osteogenic differentiation and in vivo new bone formation using the alendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarial defect. Aln/PMS system significantly enhanced in vitro osteogenic differentiation and maturation, showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin and osteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMS system increased mineralization and bone matrix formation compared to PMS alone. This study demonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbit adipose-derived stem cells osteogenesis and bone regeneration.

Original language	English
Pages (from-to)	277-286
Number of pages	10
Journal	Journal of Industrial and Engineering Chemistry
Volume	52
DOIs	https://doi.org/10.1016/j.jiec.2017.03.057
Publication status	Published - 2017 Aug 25

Bibliographical note

Publisher Copyright:
© 2017 The Korean Society of Industrial and Engineering Chemistry

Keywords

Alendronate (Aln)
Bone regeneration
Calvarial defect model
Porous PLGA microspheres (PMSs)
Rabbit adipose-derived stem cells (rADSCs)

ASJC Scopus subject areas

General Chemical Engineering

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10.1016/j.jiec.2017.03.057

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title = "Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model",

abstract = "In this study, we evaluated in vitro osteogenic differentiation and in vivo new bone formation using the alendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarial defect. Aln/PMS system significantly enhanced in vitro osteogenic differentiation and maturation, showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin and osteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMS system increased mineralization and bone matrix formation compared to PMS alone. This study demonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbit adipose-derived stem cells osteogenesis and bone regeneration.",

keywords = "Alendronate (Aln), Bone regeneration, Calvarial defect model, Porous PLGA microspheres (PMSs), Rabbit adipose-derived stem cells (rADSCs)",

author = "Lee, {Jae Yong} and Kim, {Sung Eun} and Yun, {Young Pil} and Choi, {Sung Wook} and Jeon, {Daniel I.} and Kim, {Hak Jun} and Kyeongsoon Park and Song, {Hae Ryong}",

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year = "2017",

month = aug,

day = "25",

doi = "10.1016/j.jiec.2017.03.057",

language = "English",

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T1 - Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

AU - Lee, Jae Yong

AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Choi, Sung Wook

AU - Jeon, Daniel I.

AU - Kim, Hak Jun

AU - Park, Kyeongsoon

AU - Song, Hae Ryong

PY - 2017/8/25

Y1 - 2017/8/25

N2 - In this study, we evaluated in vitro osteogenic differentiation and in vivo new bone formation using the alendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarial defect. Aln/PMS system significantly enhanced in vitro osteogenic differentiation and maturation, showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin and osteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMS system increased mineralization and bone matrix formation compared to PMS alone. This study demonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbit adipose-derived stem cells osteogenesis and bone regeneration.

AB - In this study, we evaluated in vitro osteogenic differentiation and in vivo new bone formation using the alendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarial defect. Aln/PMS system significantly enhanced in vitro osteogenic differentiation and maturation, showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin and osteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMS system increased mineralization and bone matrix formation compared to PMS alone. This study demonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbit adipose-derived stem cells osteogenesis and bone regeneration.

KW - Alendronate (Aln)

KW - Bone regeneration

KW - Calvarial defect model

KW - Porous PLGA microspheres (PMSs)

KW - Rabbit adipose-derived stem cells (rADSCs)

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U2 - 10.1016/j.jiec.2017.03.057

DO - 10.1016/j.jiec.2017.03.057

M3 - Article

AN - SCOPUS:85017448641

SN - 1226-086X

VL - 52

SP - 277

EP - 286

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

ER -

Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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