Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers

Sung Eun Kim; Young Pil Yun; Yun Kyung Han; Deok Won Lee; Joo Young Ohe; Baek Soo Lee; Hae Ryong Song; Kyeongsoon Park; Byung Joon Choi

doi:10.1016/j.carbpol.2013.08.053

Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers

Sung Eun Kim, Young Pil Yun, Yun Kyung Han, Deok Won Lee, Joo Young Ohe, Baek Soo Lee, Hae Ryong Song, Kyeongsoon Park, Byung Joon Choi

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs.

Original language	English
Pages (from-to)	700-709
Number of pages	10
Journal	Carbohydrate Polymers
Volume	99
DOIs	https://doi.org/10.1016/j.carbpol.2013.08.053
Publication status	Published - 2014

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of Korea ( HI11C038 8) and a KBSI grant ( T33513 ) to KP.

Keywords

Bone morphogenic protein-2 (BMP-2)
Osteogenesis induction
PCL fiber
Periodontal ligament cells (PDLCs)

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.carbpol.2013.08.053

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title = "Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers",

abstract = "The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs.",

keywords = "Bone morphogenic protein-2 (BMP-2), Osteogenesis induction, PCL fiber, Periodontal ligament cells (PDLCs)",

author = "Kim, {Sung Eun} and Yun, {Young Pil} and Han, {Yun Kyung} and Lee, {Deok Won} and Ohe, {Joo Young} and Lee, {Baek Soo} and Song, {Hae Ryong} and Kyeongsoon Park and Choi, {Byung Joon}",

note = "Funding Information: This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of Korea ( HI11C038 8) and a KBSI grant ( T33513 ) to KP. ",

year = "2014",

doi = "10.1016/j.carbpol.2013.08.053",

language = "English",

volume = "99",

pages = "700--709",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier Ltd",

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T1 - Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers

AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Han, Yun Kyung

AU - Lee, Deok Won

AU - Ohe, Joo Young

AU - Lee, Baek Soo

AU - Song, Hae Ryong

AU - Park, Kyeongsoon

AU - Choi, Byung Joon

N1 - Funding Information: This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of Korea ( HI11C038 8) and a KBSI grant ( T33513 ) to KP.

PY - 2014

Y1 - 2014

N2 - The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs.

AB - The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs.

KW - Bone morphogenic protein-2 (BMP-2)

KW - Osteogenesis induction

KW - PCL fiber

KW - Periodontal ligament cells (PDLCs)

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M3 - Article

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AN - SCOPUS:84884328944

SN - 0144-8617

VL - 99

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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