Zirconia has recently gained importance as a dental implant material due to its favourable properties. The present study aimed to evaluate the osteogenic potential of Non Thermal Atmospheric Pressure Plasma treated YTZP using O2 and N2 gases. The proliferation and differentiation of osteoblastic cell-line MC3T3-E1 was tested using dielectric barrier discharge plasma as a new technique. YTZP discs were divided into control (untreated) and plasma (O2 or N2) treated groups on which MC3T3-E1 osteoblastic cells were seeded. The specimens were tested for cell cytotoxicity and differentiation and surface characteristics were analysed using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray spectroscopy (EDS) and contact angle analysis. Surface analysis indicated generation of zirconium oxide nanoparticles on the discs and the contact angle decreased slightly on treatment with O2 and N2 gas. XPS and EDS analysis revealed the structural effects on YTZP surface due to plasma treatment. Alizarin Red Staining indicated increased osteogenic response on plasma coated YTZP discs (N2Effect > O2Effect). The overall effect of plasma surface treatment was positive for MC3T3-E1 osteoblasts. The results suggest that plasma treatment improves the YTZP surface and is favourable for osteogenesis.
Bibliographical noteFunding Information:
This work was supported by a grant from the National Research Foundation of Korea (NRF-2016K1A4A3914113 and NRF-2015R1C1A2A01054137) funded by the Ministry of Science, ICT and Future Planning (MSIP) of Korean Government. We thank the Neobiotech® Company (Seoul, South Korea) for kindly providing the Zirconia discs. We also thank Professor In Seok Song (Oral and Maxillofacial Surgery Department, Korea University) for his guidance and Ju Sung Kim (Kwangwoon University) for assisting in SEM analysis.
© 2017 American Scientific Publishers. All rights reserved.
- Dental implants
- Dielectric barrier discharge (DBD)
- MC3T3-E1 osteoblasts
- Non-thermal biocompatible atmospheric plasma (NBP)
- YTZP (yttria stabilized tetragonal zirconia)
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biomedical Engineering