Overexpression of a plant U-box gene TaPUB4 confers drought stress tolerance in Arabidopsis thaliana

Jae Ho Kim, Moon Seok Kim, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Drought stress frequently results in significant reductions in crop production and yield. Plant U-box proteins (PUB) play a key role in the response to abiotic stress. Despite extensive characterization of PUB in model plants, their roles in wheat abiotic stress response remains unknown. In this study, we identified the physiological function of TaPUB4, a gene encoding the U-box and nuclear localization domains. The transcription level of TaPUB4 was induced by drought (mannitol) and abscisic acid. TaPUB4 displays E3 ubiquitin ligase activity and is located in the nucleus. Overexpression of TaPUB4 in Arabidopsis plants enhanced sensitivity with under ABA condition during early seedling developmental stages. In addition, the stomatal conductance of TaPUB4 was closer to that of WT under ABA conditions. Moreover, TaPUB4 facilitated stomatal response to elevated CO2 emission rates under ABA conditions. TaPUB4-overexpressing Arabidopsis, on the other hand, was more resistant to drought stress in plant development, demonstrating that TaPUB4 positively regulates drought-mediated control of plant growth. Moreover, the ectopic expression of the TaPUB4 gene was significant influential in drought sensitive metrics including survival rate, chlorophyll content, water loss, proline content and the expression of drought stress-response genes. Collectively, our results demonstrate that TaPUB4 may regulate drought stress response and ABA conditions.

Original languageEnglish
Pages (from-to)596-607
Number of pages12
JournalPlant Physiology and Biochemistry
Volume196
DOIs
Publication statusPublished - 2023 Mar

Bibliographical note

Funding Information:
This work was conducted with the support of the “ Basic Science Research Program ” through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning ( 2017R1D1A1B06030349 ), and a grant from the Korea University, Republic of Korea . The authors would like to thank Prof. Jong Yun Kim for providing CIRA S-3.

Publisher Copyright:
© 2023 Elsevier Masson SAS

Keywords

  • Arabidopsis
  • Drought
  • Plant U-box E3 ligase
  • TaPUB4
  • Ubiquitination
  • Wheat

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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