Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang Woo Lee; Bu Soo Park; Eun Sil Choi; Min Kyu Oh

doi:10.2478/s11814-010-0100-3

Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang Woo Lee, Bu Soo Park, Eun Sil Choi, Min Kyu Oh

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

Original language	English
Pages (from-to)	587-589
Number of pages	3
Journal	Korean Journal of Chemical Engineering
Volume	27
Issue number	2
DOIs	https://doi.org/10.2478/s11814-010-0100-3
Publication status	Published - 2010 Mar

Keywords

Metabolic Engineering
S-adenosyl-L-methionine
Saccharomyces cerevisiae sake

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.2478/s11814-010-0100-3

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title = "Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6",

abstract = "In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.",

keywords = "Metabolic Engineering, S-adenosyl-L-methionine, Saccharomyces cerevisiae sake",

author = "Lee, {Sang Woo} and Park, {Bu Soo} and Choi, {Eun Sil} and Oh, {Min Kyu}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-331-D00133) and Yang Young Foundation.",

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doi = "10.2478/s11814-010-0100-3",

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T1 - Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

AU - Lee, Sang Woo

AU - Park, Bu Soo

AU - Choi, Eun Sil

AU - Oh, Min Kyu

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-331-D00133) and Yang Young Foundation.

PY - 2010/3

Y1 - 2010/3

N2 - In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

AB - In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

KW - Metabolic Engineering

KW - S-adenosyl-L-methionine

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Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Abstract

Keywords

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