Overexpression of human erythropoietin (EPO) affects plant morphologies: Retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis

Ban Yoon Cheon, Hae Jin Kim, Kyung Hee Oh, Sung Chul Bahn, Ji Hoon Ahn, Jang Won Choi, Sung Han Ok, Jung Myung Bae, Jeong Sheop Shin

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and Arabidopsis.

Original languageEnglish
Pages (from-to)541-549
Number of pages9
JournalTransgenic Research
Volume13
Issue number6
DOIs
Publication statusPublished - 2004 Dec

Keywords

  • Arabidopsis thaliana
  • Nicotiana tabacum
  • dwarfism
  • erythropoietin (EPO)
  • male sterility
  • overexpression
  • phenotypic change

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

Fingerprint

Dive into the research topics of 'Overexpression of human erythropoietin (EPO) affects plant morphologies: Retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis'. Together they form a unique fingerprint.

Cite this