Abstract
Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and Arabidopsis.
Original language | English |
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Pages (from-to) | 541-549 |
Number of pages | 9 |
Journal | Transgenic Research |
Volume | 13 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2004 Dec |
Keywords
- Arabidopsis thaliana
- Nicotiana tabacum
- dwarfism
- erythropoietin (EPO)
- male sterility
- overexpression
- phenotypic change
ASJC Scopus subject areas
- Biotechnology
- Animal Science and Zoology
- Agronomy and Crop Science
- Genetics