Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

Sujon Sarowar; Young Jin Kim; Ki Deok Kim; Byung Kook Hwang; Sung Han Ok; Jeong Sheop Shin

doi:10.1007/s00299-008-0653-3

Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

Sujon Sarowar, Young Jin Kim, Ki Deok Kim, Byung Kook Hwang, Sung Han Ok, Jeong Sheop Shin

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

The lipid signal is essential for the activation of plant defense responses, but downstream components of the signaling pathway are still poorly defined. To investigate the biological functions of pepper lipid transfer protein (LTP), we carried out virus-induced gene silencing (VIGS) in pepper, constitutive expression of CALTPs and grafting experiments in the tobacco plant. Suppression of endogenous CALTPI and CALTPII by VIGS, respectively, resulted in enhanced susceptibility to Xanthomonas campestris pv. vescatoria and pepper mosaic mottle virus in pepper. On the other hand, the constitutive expression of CALTPI and CALTPII genes in tobacco plants showed enhanced resistance to oomycete pathogen, Phytophthora nicotianae and bacterial pathogen, Pseudomonas syringae pv. tabaci. Enhanced resistance is found to be associated with the enhanced CALTP transcript levels in the independent transgenic CALTPI or II tobacco lines. Induced resistance responses in grafted scion leaves revealed that LTP plays a role in long-distance systemic signaling in plants.

Original language	English
Pages (from-to)	419-427
Number of pages	9
Journal	Plant Cell Reports
Volume	28
Issue number	3
DOIs	https://doi.org/10.1007/s00299-008-0653-3
Publication status	Published - 2009 Mar

Keywords

Disease resistance
Lipid transfer protein
Phytophthora nicotianae
Pseudomonas syringae pv. tabaci
Transgenic plant
Virus-induced gene silencing

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s00299-008-0653-3

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@article{7d7dbdd6723f4e1bb1566f4ea7cf5b4e,

title = "Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco",

abstract = "The lipid signal is essential for the activation of plant defense responses, but downstream components of the signaling pathway are still poorly defined. To investigate the biological functions of pepper lipid transfer protein (LTP), we carried out virus-induced gene silencing (VIGS) in pepper, constitutive expression of CALTPs and grafting experiments in the tobacco plant. Suppression of endogenous CALTPI and CALTPII by VIGS, respectively, resulted in enhanced susceptibility to Xanthomonas campestris pv. vescatoria and pepper mosaic mottle virus in pepper. On the other hand, the constitutive expression of CALTPI and CALTPII genes in tobacco plants showed enhanced resistance to oomycete pathogen, Phytophthora nicotianae and bacterial pathogen, Pseudomonas syringae pv. tabaci. Enhanced resistance is found to be associated with the enhanced CALTP transcript levels in the independent transgenic CALTPI or II tobacco lines. Induced resistance responses in grafted scion leaves revealed that LTP plays a role in long-distance systemic signaling in plants.",

keywords = "Disease resistance, Lipid transfer protein, Phytophthora nicotianae, Pseudomonas syringae pv. tabaci, Transgenic plant, Virus-induced gene silencing",

author = "Sujon Sarowar and Kim, {Young Jin} and Kim, {Ki Deok} and Hwang, {Byung Kook} and Ok, {Sung Han} and Shin, {Jeong Sheop}",

note = "Funding Information: Acknowledgments We thank Dr. S.P. Dinesh-Kumar (Yale University) for the pTRV1 and pTRV2 vectors. This study was supported by the special grant from the Agricultural R&D Promotion Center funded by the Ministry of Agriculture and Forestry, by a grant (code CG3112) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program and by a grant from the SIGNET (R11-2003-008-04006) and SIGNET (R11-2003-008-02006) funded by the Ministry of Science and Technology (MOST), Republic of Korea.",

year = "2009",

month = mar,

doi = "10.1007/s00299-008-0653-3",

language = "English",

volume = "28",

pages = "419--427",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

number = "3",

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T1 - Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

AU - Sarowar, Sujon

AU - Kim, Young Jin

AU - Kim, Ki Deok

AU - Hwang, Byung Kook

AU - Ok, Sung Han

AU - Shin, Jeong Sheop

N1 - Funding Information: Acknowledgments We thank Dr. S.P. Dinesh-Kumar (Yale University) for the pTRV1 and pTRV2 vectors. This study was supported by the special grant from the Agricultural R&D Promotion Center funded by the Ministry of Agriculture and Forestry, by a grant (code CG3112) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program and by a grant from the SIGNET (R11-2003-008-04006) and SIGNET (R11-2003-008-02006) funded by the Ministry of Science and Technology (MOST), Republic of Korea.

PY - 2009/3

Y1 - 2009/3

N2 - The lipid signal is essential for the activation of plant defense responses, but downstream components of the signaling pathway are still poorly defined. To investigate the biological functions of pepper lipid transfer protein (LTP), we carried out virus-induced gene silencing (VIGS) in pepper, constitutive expression of CALTPs and grafting experiments in the tobacco plant. Suppression of endogenous CALTPI and CALTPII by VIGS, respectively, resulted in enhanced susceptibility to Xanthomonas campestris pv. vescatoria and pepper mosaic mottle virus in pepper. On the other hand, the constitutive expression of CALTPI and CALTPII genes in tobacco plants showed enhanced resistance to oomycete pathogen, Phytophthora nicotianae and bacterial pathogen, Pseudomonas syringae pv. tabaci. Enhanced resistance is found to be associated with the enhanced CALTP transcript levels in the independent transgenic CALTPI or II tobacco lines. Induced resistance responses in grafted scion leaves revealed that LTP plays a role in long-distance systemic signaling in plants.

AB - The lipid signal is essential for the activation of plant defense responses, but downstream components of the signaling pathway are still poorly defined. To investigate the biological functions of pepper lipid transfer protein (LTP), we carried out virus-induced gene silencing (VIGS) in pepper, constitutive expression of CALTPs and grafting experiments in the tobacco plant. Suppression of endogenous CALTPI and CALTPII by VIGS, respectively, resulted in enhanced susceptibility to Xanthomonas campestris pv. vescatoria and pepper mosaic mottle virus in pepper. On the other hand, the constitutive expression of CALTPI and CALTPII genes in tobacco plants showed enhanced resistance to oomycete pathogen, Phytophthora nicotianae and bacterial pathogen, Pseudomonas syringae pv. tabaci. Enhanced resistance is found to be associated with the enhanced CALTP transcript levels in the independent transgenic CALTPI or II tobacco lines. Induced resistance responses in grafted scion leaves revealed that LTP plays a role in long-distance systemic signaling in plants.

KW - Disease resistance

KW - Lipid transfer protein

KW - Phytophthora nicotianae

KW - Pseudomonas syringae pv. tabaci

KW - Transgenic plant

KW - Virus-induced gene silencing

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U2 - 10.1007/s00299-008-0653-3

DO - 10.1007/s00299-008-0653-3

M3 - Article

C2 - 19089429

AN - SCOPUS:61449207847

SN - 0721-7714

VL - 28

SP - 419

EP - 427

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 3

ER -

Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

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