Overexpression of malic enzyme in the larval stage extends Drosophila lifespan

Gye Hyeong Kim; Young Eun Lee; Gun Ho Lee; Youn Ho Cho; Young Nam Lee; Yeogil Jang; Donggi Paik; Joong Jean Park

doi:10.1016/j.bbrc.2014.12.020

Overexpression of malic enzyme in the larval stage extends Drosophila lifespan

Gye Hyeong Kim, Young Eun Lee, Gun Ho Lee, Youn Ho Cho, Young Nam Lee, Yeogil Jang, Donggi Paik, Joong Jean Park

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP⁺ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.

Original language	English
Pages (from-to)	676-682
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	456
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2014.12.020
Publication status	Published - 2015 Jan 9
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.

Keywords

Drosophila
Longevity
Malic enzyme
Pyruvate
ROS

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2014.12.020

Cite this

@article{5112f6a2769c4228b9376d668e6bd841,

title = "Overexpression of malic enzyme in the larval stage extends Drosophila lifespan",

abstract = "Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.",

keywords = "Drosophila, Longevity, Malic enzyme, Pyruvate, ROS",

author = "Kim, {Gye Hyeong} and Lee, {Young Eun} and Lee, {Gun Ho} and Cho, {Youn Ho} and Lee, {Young Nam} and Yeogil Jang and Donggi Paik and Park, {Joong Jean}",

year = "2015",

month = jan,

day = "9",

doi = "10.1016/j.bbrc.2014.12.020",

language = "English",

volume = "456",

pages = "676--682",

journal = "Biochemical and biophysical research communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Overexpression of malic enzyme in the larval stage extends Drosophila lifespan

AU - Kim, Gye Hyeong

AU - Lee, Young Eun

AU - Lee, Gun Ho

AU - Cho, Youn Ho

AU - Lee, Young Nam

AU - Jang, Yeogil

AU - Paik, Donggi

AU - Park, Joong Jean

PY - 2015/1/9

Y1 - 2015/1/9

N2 - Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.

AB - Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.

KW - Drosophila

KW - Longevity

KW - Malic enzyme

KW - Pyruvate

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=84919911772&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2014.12.020

DO - 10.1016/j.bbrc.2014.12.020

M3 - Article

C2 - 25511696

AN - SCOPUS:84919911772

SN - 0006-291X

VL - 456

SP - 676

EP - 682

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 2

ER -

Overexpression of malic enzyme in the larval stage extends Drosophila lifespan

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this