Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging

Valentina A. Shchedrina; Gerd Vorbrüggen; Byung Cheon Lee; Hwa Young Kim; Hadise Kabil; Lawrence G. Harshman; Vadim N. Gladyshev

doi:10.1016/j.mad.2009.04.003

Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging

Valentina A. Shchedrina, Gerd Vorbrüggen, Byung Cheon Lee, Hwa Young Kim, Hadise Kabil, Lawrence G. Harshman, Vadim N. Gladyshev

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.

Original language	English
Pages (from-to)	429-443
Number of pages	15
Journal	Mechanisms of Ageing and Development
Volume	130
Issue number	7
DOIs	https://doi.org/10.1016/j.mad.2009.04.003
Publication status	Published - 2009 Jul
Externally published	Yes

Bibliographical note

Funding Information:
The authors are very grateful to Dr. Daryl A. Travnicek (Department of Statistics, University of Nebraska-Lincoln, USA) for help with statistical analyses. This work was supported by the NIH (National Institute of Health) grants AG021518 (to VNG) and DK074136 (to LGH), and the KOSEF (Korea Science and Engineering Foundation) grant M10642040001-07N4204-00111 (to HYK).

Keywords

Aging
Drosophila melanogaster
Lifespan
Methionine sulfoxide reductase
Protein repair

ASJC Scopus subject areas

Ageing
Developmental Biology

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10.1016/j.mad.2009.04.003

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title = "Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging",

abstract = "Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.",

keywords = "Aging, Drosophila melanogaster, Lifespan, Methionine sulfoxide reductase, Protein repair",

author = "Shchedrina, {Valentina A.} and Gerd Vorbr{\"u}ggen and Lee, {Byung Cheon} and Kim, {Hwa Young} and Hadise Kabil and Harshman, {Lawrence G.} and Gladyshev, {Vadim N.}",

note = "Funding Information: The authors are very grateful to Dr. Daryl A. Travnicek (Department of Statistics, University of Nebraska-Lincoln, USA) for help with statistical analyses. This work was supported by the NIH (National Institute of Health) grants AG021518 (to VNG) and DK074136 (to LGH), and the KOSEF (Korea Science and Engineering Foundation) grant M10642040001-07N4204-00111 (to HYK). ",

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AU - Vorbrüggen, Gerd

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AU - Kim, Hwa Young

AU - Kabil, Hadise

AU - Harshman, Lawrence G.

AU - Gladyshev, Vadim N.

N1 - Funding Information: The authors are very grateful to Dr. Daryl A. Travnicek (Department of Statistics, University of Nebraska-Lincoln, USA) for help with statistical analyses. This work was supported by the NIH (National Institute of Health) grants AG021518 (to VNG) and DK074136 (to LGH), and the KOSEF (Korea Science and Engineering Foundation) grant M10642040001-07N4204-00111 (to HYK).

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N2 - Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.

AB - Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.

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Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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