Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization

Hyeong Min Han; In Jung Kim; Eun Ju Yun; Jae Won Lee; Yoonho Cho; Yong Su Jin; Kyoung Heon Kim

doi:10.1007/s12010-020-03409-4

Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization

Hyeong Min Han, In Jung Kim, Eun Ju Yun, Jae Won Lee, Yoonho Cho, Yong Su Jin, Kyoung Heon Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Colanic acid (CA) is one of the major bacterial exopolysaccharides. Due to its biological activities, CA has a significant commercial value. However, the cultivation conditions have not been optimized for the large-scale production of CA. Here, we constructed a CA-overproducing Escherichia coli strain (ΔwaaF) and statistically optimized its culture media for maximum CA production. Glucose and tryptone were found the optimal carbon and nitrogen sources, respectively. Fractional factorial design indicated tryptone and Na₂HPO₄ as the critical nutrients for CA production. Through further optimization, we achieved a maximum CA production of 1910.0 mg/L, which is approximately 12-fold higher than the amount obtained using the non-optimized medium initially used. The predicted value of CA production was comparable with experimental value (2052.8 mg/L) under the optimized conditions. This study constitutes a successful demonstration of media optimization for increased CA production, and paves the way for future research for achieving large-scale CA production.

Original language	English
Pages (from-to)	111-127
Number of pages	17
Journal	Applied Biochemistry and Biotechnology
Volume	193
Issue number	1
DOIs	https://doi.org/10.1007/s12010-020-03409-4
Publication status	Published - 2021 Jan

Keywords

Colanic acid
Escherichia coli ΔwaaF
Exopolysaccharide
Fractional factorial design
Media optimization
Response surface methodology
Steepest ascent method

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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10.1007/s12010-020-03409-4

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title = "Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization",

abstract = "Colanic acid (CA) is one of the major bacterial exopolysaccharides. Due to its biological activities, CA has a significant commercial value. However, the cultivation conditions have not been optimized for the large-scale production of CA. Here, we constructed a CA-overproducing Escherichia coli strain (ΔwaaF) and statistically optimized its culture media for maximum CA production. Glucose and tryptone were found the optimal carbon and nitrogen sources, respectively. Fractional factorial design indicated tryptone and Na2HPO4 as the critical nutrients for CA production. Through further optimization, we achieved a maximum CA production of 1910.0 mg/L, which is approximately 12-fold higher than the amount obtained using the non-optimized medium initially used. The predicted value of CA production was comparable with experimental value (2052.8 mg/L) under the optimized conditions. This study constitutes a successful demonstration of media optimization for increased CA production, and paves the way for future research for achieving large-scale CA production.",

keywords = "Colanic acid, Escherichia coli ΔwaaF, Exopolysaccharide, Fractional factorial design, Media optimization, Response surface methodology, Steepest ascent method",

author = "Han, {Hyeong Min} and Kim, {In Jung} and Yun, {Eun Ju} and Lee, {Jae Won} and Yoonho Cho and Jin, {Yong Su} and Kim, {Kyoung Heon}",

note = "Funding Information: We acknowledge the facility support of the Institute of Biomedical and Food Safety at CJ Food Safety Hall, Korea University. Funding Information: This work was supported by the Mid-career Researcher Program from the National Research Foundation of Korea (2020R1A2B5B02002631). IJK acknowledges the grant support from the Research Fellow Program through NRF (2017R1A6A3A11030496). Acknowledgments Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

month = jan,

doi = "10.1007/s12010-020-03409-4",

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T1 - Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization

AU - Han, Hyeong Min

AU - Kim, In Jung

AU - Yun, Eun Ju

AU - Lee, Jae Won

AU - Cho, Yoonho

AU - Jin, Yong Su

AU - Kim, Kyoung Heon

N1 - Funding Information: We acknowledge the facility support of the Institute of Biomedical and Food Safety at CJ Food Safety Hall, Korea University. Funding Information: This work was supported by the Mid-career Researcher Program from the National Research Foundation of Korea (2020R1A2B5B02002631). IJK acknowledges the grant support from the Research Fellow Program through NRF (2017R1A6A3A11030496). Acknowledgments Publisher Copyright: © 2020, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2021/1

Y1 - 2021/1

N2 - Colanic acid (CA) is one of the major bacterial exopolysaccharides. Due to its biological activities, CA has a significant commercial value. However, the cultivation conditions have not been optimized for the large-scale production of CA. Here, we constructed a CA-overproducing Escherichia coli strain (ΔwaaF) and statistically optimized its culture media for maximum CA production. Glucose and tryptone were found the optimal carbon and nitrogen sources, respectively. Fractional factorial design indicated tryptone and Na2HPO4 as the critical nutrients for CA production. Through further optimization, we achieved a maximum CA production of 1910.0 mg/L, which is approximately 12-fold higher than the amount obtained using the non-optimized medium initially used. The predicted value of CA production was comparable with experimental value (2052.8 mg/L) under the optimized conditions. This study constitutes a successful demonstration of media optimization for increased CA production, and paves the way for future research for achieving large-scale CA production.

AB - Colanic acid (CA) is one of the major bacterial exopolysaccharides. Due to its biological activities, CA has a significant commercial value. However, the cultivation conditions have not been optimized for the large-scale production of CA. Here, we constructed a CA-overproducing Escherichia coli strain (ΔwaaF) and statistically optimized its culture media for maximum CA production. Glucose and tryptone were found the optimal carbon and nitrogen sources, respectively. Fractional factorial design indicated tryptone and Na2HPO4 as the critical nutrients for CA production. Through further optimization, we achieved a maximum CA production of 1910.0 mg/L, which is approximately 12-fold higher than the amount obtained using the non-optimized medium initially used. The predicted value of CA production was comparable with experimental value (2052.8 mg/L) under the optimized conditions. This study constitutes a successful demonstration of media optimization for increased CA production, and paves the way for future research for achieving large-scale CA production.

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KW - Response surface methodology

KW - Steepest ascent method

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Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization

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