Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction

Haein Keum; Guyoung Kang; Namhyun Chung

doi:10.1007/s13765-017-0305-9

Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction

Haein Keum, Guyoung Kang, Namhyun Chung

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The efficiency to remove polycyclic aromatic hydrocarbons (PAHs) was determined using a hemoglobin-catalyzed biocatalytic reaction. The present study employed pyrene as a model of PAHs to study its oxidative removal in the presence of H₂O₂ and hemoglobin in mass ratio of 3:1. The extent of pyrene removal reached up to 91.1% in the presence of H₂O₂ and hemoglobin. However, the extent of pyrene removal was 21.3% in the presence of H₂O₂ only. The results indicate that pyrene removal might be due to pyrene oxidation by the biocatalytic reaction. Overall, this study demonstrated that hemoglobin-catalyzed biocatalytic reactions could remediate pyrene effectively.

Original language	English
Pages (from-to)	505-508
Number of pages	4
Journal	Applied Biological Chemistry
Volume	60
Issue number	5
DOIs	https://doi.org/10.1007/s13765-017-0305-9
Publication status	Published - 2017 Oct 1

Bibliographical note

Funding Information:
Acknowledgment This study was supported by the Korea Basic Science Institute (KBSI) and was partially supported by a grant from Hankuk University of Foreign Studies.

Publisher Copyright:
© 2017, The Korean Society for Applied Biological Chemistry.

Keywords

Biocatalytic reaction
Hemoglobin
Hydrogen peroxide
Oxidation
Pyrene

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Organic Chemistry

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10.1007/s13765-017-0305-9

Cite this

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title = "Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction",

abstract = "The efficiency to remove polycyclic aromatic hydrocarbons (PAHs) was determined using a hemoglobin-catalyzed biocatalytic reaction. The present study employed pyrene as a model of PAHs to study its oxidative removal in the presence of H2O2 and hemoglobin in mass ratio of 3:1. The extent of pyrene removal reached up to 91.1% in the presence of H2O2 and hemoglobin. However, the extent of pyrene removal was 21.3% in the presence of H2O2 only. The results indicate that pyrene removal might be due to pyrene oxidation by the biocatalytic reaction. Overall, this study demonstrated that hemoglobin-catalyzed biocatalytic reactions could remediate pyrene effectively.",

keywords = "Biocatalytic reaction, Hemoglobin, Hydrogen peroxide, Oxidation, Pyrene",

author = "Haein Keum and Guyoung Kang and Namhyun Chung",

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T1 - Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction

AU - Keum, Haein

AU - Kang, Guyoung

AU - Chung, Namhyun

N1 - Funding Information: Acknowledgment This study was supported by the Korea Basic Science Institute (KBSI) and was partially supported by a grant from Hankuk University of Foreign Studies. Publisher Copyright: © 2017, The Korean Society for Applied Biological Chemistry.

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N2 - The efficiency to remove polycyclic aromatic hydrocarbons (PAHs) was determined using a hemoglobin-catalyzed biocatalytic reaction. The present study employed pyrene as a model of PAHs to study its oxidative removal in the presence of H2O2 and hemoglobin in mass ratio of 3:1. The extent of pyrene removal reached up to 91.1% in the presence of H2O2 and hemoglobin. However, the extent of pyrene removal was 21.3% in the presence of H2O2 only. The results indicate that pyrene removal might be due to pyrene oxidation by the biocatalytic reaction. Overall, this study demonstrated that hemoglobin-catalyzed biocatalytic reactions could remediate pyrene effectively.

AB - The efficiency to remove polycyclic aromatic hydrocarbons (PAHs) was determined using a hemoglobin-catalyzed biocatalytic reaction. The present study employed pyrene as a model of PAHs to study its oxidative removal in the presence of H2O2 and hemoglobin in mass ratio of 3:1. The extent of pyrene removal reached up to 91.1% in the presence of H2O2 and hemoglobin. However, the extent of pyrene removal was 21.3% in the presence of H2O2 only. The results indicate that pyrene removal might be due to pyrene oxidation by the biocatalytic reaction. Overall, this study demonstrated that hemoglobin-catalyzed biocatalytic reactions could remediate pyrene effectively.

KW - Biocatalytic reaction

KW - Hemoglobin

KW - Hydrogen peroxide

KW - Oxidation

KW - Pyrene

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JO - Applied Biological Chemistry

JF - Applied Biological Chemistry

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Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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