TY - JOUR
T1 - Oxidative damages in the DNA, lipids, and proteins of rats exposed to isofluranes and alcohols
AU - Kim, Heezoo
AU - Oh, Eunha
AU - Im, Hosub
AU - Mun, Joohee
AU - Yang, Minho
AU - Khim, Jin Young
AU - Lee, Eunil
AU - Lim, Sang Ho
AU - Kong, Myoung Hoon
AU - Lee, Mikyoung
AU - Sul, Donggeun
N1 - Funding Information:
This research was supported by the grant of Medical Research Center for Environmental Toxico-Genomics & Proteomics and funded by Korea Science and Engineering Foundations and ministry of Science & Technology.
PY - 2006/3/15
Y1 - 2006/3/15
N2 - DNA damage, lipid peroxidation and protein oxidation were evaluated in rats exposed to a 1% isoflurane atmosphere with or without alcohol administration (administrated by gastric intubation at 4 g/kg body weight as a 50% solution). Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the lymphocytes, spleen, bone marrow, brain, livers and lung of rats exposed to 1% isoflurane for 30 or 60 min with/without ethanol. Levels of malondialdehydes (MDA), a metabolite of lipid peroxidation, were determined in plasma and tissues. Carbonyl contents were also analyzed to determine levels of protein oxidation in plasma and tissues. Levels of DNA damage in lymphocytes, bone marrow, and the organ tissues of rats exposed to isoflurane were found to increase time dependently, and alcohol increased DNA damage. Lipid peroxidation and protein oxidation results showed patterns that differed from those of DNA damage. Levels of MDA in plasma, bone marrow, spleen, and the livers of rats exposed to isoflurane with/without ethanol were found to be time dependently increased, but this was not observed in the brain or lung. However, protein oxidation levels were significantly increased in the plasma, brains, and lungs of rats exposed to isoflurane, and exposure to isoflurane and alcohol, significantly increased these levels in plasma and brain. The present study demonstrates that isoflurane exposure results in significant DNA damage in rat lymphocytes, bone marrow, spleen, brain, livers, and lung. Moreover, alcohol was found to be as a strong inducer of DNA damage, lipid peroxidation and protein oxidation. However, no evidence in association between DNA damage, lipid peroxidation and protein oxidation was found. Regarding the effects of isoflurane and alcohol on oxidative damages, single strand DNA damages may be a useful biomarkers and blood cells and plasma appear to be more sensitive targets to oxidative damage than other tissues.
AB - DNA damage, lipid peroxidation and protein oxidation were evaluated in rats exposed to a 1% isoflurane atmosphere with or without alcohol administration (administrated by gastric intubation at 4 g/kg body weight as a 50% solution). Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the lymphocytes, spleen, bone marrow, brain, livers and lung of rats exposed to 1% isoflurane for 30 or 60 min with/without ethanol. Levels of malondialdehydes (MDA), a metabolite of lipid peroxidation, were determined in plasma and tissues. Carbonyl contents were also analyzed to determine levels of protein oxidation in plasma and tissues. Levels of DNA damage in lymphocytes, bone marrow, and the organ tissues of rats exposed to isoflurane were found to increase time dependently, and alcohol increased DNA damage. Lipid peroxidation and protein oxidation results showed patterns that differed from those of DNA damage. Levels of MDA in plasma, bone marrow, spleen, and the livers of rats exposed to isoflurane with/without ethanol were found to be time dependently increased, but this was not observed in the brain or lung. However, protein oxidation levels were significantly increased in the plasma, brains, and lungs of rats exposed to isoflurane, and exposure to isoflurane and alcohol, significantly increased these levels in plasma and brain. The present study demonstrates that isoflurane exposure results in significant DNA damage in rat lymphocytes, bone marrow, spleen, brain, livers, and lung. Moreover, alcohol was found to be as a strong inducer of DNA damage, lipid peroxidation and protein oxidation. However, no evidence in association between DNA damage, lipid peroxidation and protein oxidation was found. Regarding the effects of isoflurane and alcohol on oxidative damages, single strand DNA damages may be a useful biomarkers and blood cells and plasma appear to be more sensitive targets to oxidative damage than other tissues.
KW - Alcohol
KW - Bone marrow
KW - Brain
KW - Comet assay
KW - DNA damage
KW - Isoflurane
KW - Lipid peroxidation
KW - Liver
KW - Lung
KW - Lymphocytes
KW - Malondialdehyde
KW - Plasma
KW - Protein oxidation
KW - Single strand DNA breakage
KW - Spleen
UR - http://www.scopus.com/inward/record.url?scp=33244475848&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2005.12.010
DO - 10.1016/j.tox.2005.12.010
M3 - Article
C2 - 16442689
AN - SCOPUS:33244475848
SN - 0300-483X
VL - 220
SP - 169
EP - 178
JO - Toxicology
JF - Toxicology
IS - 2-3
ER -