Oxygen transfer correlation in high cell density culture of recombinant E. coli

Chul Soo Shin; Min Sun Hong; Jeewon Lee

doi:10.1007/BF00168479

Oxygen transfer correlation in high cell density culture of recombinant E. coli

Chul Soo Shin, Min Sun Hong, Jeewon Lee

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A recombinant E. coli BL21[pET3a-T2M2] was cultivated in fed-batch cultures and cell mass increased to more than 70g/L. The volumetric oxygen transfer coefficient was estimated in a range of various fermentation parameters (agitation speed, oxygen flow rate and cell mass concentration) and finally the oxygen transfer correlation in bioreactor containing the recombinant E. coli cultures was determined as: k(L)a = 0.0195 (P(g)/V)^0.55 (V₃)^0.64 (1 + 2.12X + 0.20X²)^-0^.25.

Original language	English
Pages (from-to)	679-682
Number of pages	4
Journal	Biotechnology Techniques
Volume	10
Issue number	9
DOIs	https://doi.org/10.1007/BF00168479
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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title = "Oxygen transfer correlation in high cell density culture of recombinant E. coli",

abstract = "A recombinant E. coli BL21[pET3a-T2M2] was cultivated in fed-batch cultures and cell mass increased to more than 70g/L. The volumetric oxygen transfer coefficient was estimated in a range of various fermentation parameters (agitation speed, oxygen flow rate and cell mass concentration) and finally the oxygen transfer correlation in bioreactor containing the recombinant E. coli cultures was determined as: k(L)a = 0.0195 (P(g)/V)0.55 (V3)0.64 (1 + 2.12X + 0.20X2)-0.25.",

author = "Shin, {Chul Soo} and Hong, {Min Sun} and Jeewon Lee",

year = "1996",

doi = "10.1007/BF00168479",

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AU - Shin, Chul Soo

AU - Hong, Min Sun

AU - Lee, Jeewon

PY - 1996

Y1 - 1996

N2 - A recombinant E. coli BL21[pET3a-T2M2] was cultivated in fed-batch cultures and cell mass increased to more than 70g/L. The volumetric oxygen transfer coefficient was estimated in a range of various fermentation parameters (agitation speed, oxygen flow rate and cell mass concentration) and finally the oxygen transfer correlation in bioreactor containing the recombinant E. coli cultures was determined as: k(L)a = 0.0195 (P(g)/V)0.55 (V3)0.64 (1 + 2.12X + 0.20X2)-0.25.

AB - A recombinant E. coli BL21[pET3a-T2M2] was cultivated in fed-batch cultures and cell mass increased to more than 70g/L. The volumetric oxygen transfer coefficient was estimated in a range of various fermentation parameters (agitation speed, oxygen flow rate and cell mass concentration) and finally the oxygen transfer correlation in bioreactor containing the recombinant E. coli cultures was determined as: k(L)a = 0.0195 (P(g)/V)0.55 (V3)0.64 (1 + 2.12X + 0.20X2)-0.25.

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DO - 10.1007/BF00168479

M3 - Article

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SP - 679

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 9

ER -

Oxygen transfer correlation in high cell density culture of recombinant E. coli

Abstract

ASJC Scopus subject areas

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