Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells

Hyun A. Jo, Dahyun Hwang, Jeong Keun Kim, Young Hee Lim

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Strengthening intestinal tight junctions (TJ) provides an effective barrier from the external environment and is important for recovery from inflammatory bowel disease. Oxyresveratrol (OXY), an isomer of hydroxylated resveratrol, is isolated from many plants. The aim of this study was to investigate the effect of OXY on intestinal TJ and to elucidate the mechanism underlying the OXY-mediated increase in TJ integrity in human intestinal Caco-2 cells. OXY-treated Caco-2 cell monolayers showed decreased monolayer permeability as evaluated by paracellular transport assay. The results showed that OXY significantly increased the levels of TJ-related genes and proteins (Claudin-1, Occludin and ZO-1) compared with those of the negative control. OXY activated protein kinase C (PKC) and increased expression levels of mitogen-activated protein kinase (MAPK) genes. OXY also increased gene and protein levels of the transcription factor Cdx-2. Expression levels of TJ, PKC and Cdx-2 proteins and transepithelial electrical resistance (TEER) value decreased in OXY-treated Caco-2 cells following treatment with a pan-PKC inhibitor compared with those of the untreated control. In conclusion, OXY strengthens the integrity of the intestinal TJ barrier via activation of the PKC and MAPK pathways.

Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalFood and Chemical Toxicology
Publication statusPublished - 2017 Oct

Bibliographical note

Funding Information:
This work was supported by the High Value-added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (Grant number: 313028-3); This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2017R1A2B4011333).

Publisher Copyright:
© 2017 Elsevier Ltd


  • Intestinal permeability
  • Mitogen-activated protein kinase
  • Oxyresveratrol
  • Protein kinase C
  • Tight junctions

ASJC Scopus subject areas

  • Food Science
  • Toxicology


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