Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells

Dahyun Hwang, Hyun A. Jo, Seong Ho Ma, Young Hee Lim

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    The intestinal mucus layer plays an important role in the management of inflammatory bowel disease. The aim of this study was to investigate the effects of oxyresveratrol (OXY), an antioxidant, on the stimulation of mucin production in human LS 174T goblet cells and the underlying mechanism thereof. OXY increased MUC2 expression at both the mRNA and protein levels. By performing two-dimensional gel electrophoresis, we found that the expression of nicotinic acid phosphoribosyltransferase1 (NaPRT1) in OXY-treated LS 174T cells was greatly increased compared with that in negative control cells. In addition, the NAD+/NADH ratio was increased in proportion to OXY in LS 174T cells. The expression of NAD+-synthesis enzymes, NaPRT1, nicotinamide riboside kinase1 (NRK1) and nicotinamide mononucleotide adenylyltransferase1 (Nmnat1) was significantly increased at both the mRNA and protein levels in OXY-treated LS 174T cells. The inhibition of NaPRT1 and NRK1 did not decrease MUC2 expression after inhibiting by small interfering RNA (siRNA)-NaPRT1 and siRNA-NRK1, respectively; however, inhibition of Nmnat by an Nmnat inhibitor decreased MUC2 expression in a dose-dependent manner. In conclusion, OXY increases NAD+ levels, resulting in the stimulation of MUC2 expression in LS 174T cells. These findings present a novel role for NAD+ in stimulation of MUC2 expression.

    Original languageEnglish
    Pages (from-to)880-888
    Number of pages9
    JournalFood and Chemical Toxicology
    Volume118
    DOIs
    Publication statusPublished - 2018 Aug

    Bibliographical note

    Funding Information:
    This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) ( NRF-2017R1A2B4011333 ).

    Publisher Copyright:
    © 2018 Elsevier Ltd

    Copyright:
    Copyright 2018 Elsevier B.V., All rights reserved.

    Keywords

    • Goblet cells
    • Intestinal mucus layer
    • MUC2
    • NAD
    • Oxyresveratrol

    ASJC Scopus subject areas

    • Food Science
    • Toxicology

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