Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

Eun Soo Lee; Kyung Ok Uhm; Yun Mi Lee; Joseph Kwon; Sun Hwa Park; Kim Hyeon Soo

doi:10.1016/j.regpep.2008.05.001

Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

Eun Soo Lee
, Kyung Ok Uhm
, Yun Mi Lee
, Joseph Kwon
, Sun Hwa Park
, Kim Hyeon Soo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca²⁺/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

Original language	English
Pages (from-to)	71-74
Number of pages	4
Journal	Regulatory Peptides
Volume	151
Issue number	1-3
DOIs	https://doi.org/10.1016/j.regpep.2008.05.001
Publication status	Published - 2008 Nov 29

Bibliographical note

Funding Information:
This study was supported by a grant from Korea University and also supported by Kmep (T28021) to Jong-Soon Choi.

Keywords

ACC
AMPK
CaMKK
Calcium
Oxytocin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/j.regpep.2008.05.001

Cite this

@article{c238277bac88420683366502af099418,

title = "Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway",

abstract = "Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.",

keywords = "ACC, AMPK, CaMKK, Calcium, Oxytocin",

author = "Lee, \{Eun Soo\} and Uhm, \{Kyung Ok\} and Lee, \{Yun Mi\} and Joseph Kwon and Park, \{Sun Hwa\} and Soo, \{Kim Hyeon\}",

note = "Funding Information: This study was supported by a grant from Korea University and also supported by Kmep (T28021) to Jong-Soon Choi. ",

year = "2008",

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doi = "10.1016/j.regpep.2008.05.001",

language = "English",

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T1 - Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

AU - Lee, Eun Soo

AU - Uhm, Kyung Ok

AU - Lee, Yun Mi

AU - Kwon, Joseph

AU - Park, Sun Hwa

AU - Soo, Kim Hyeon

N1 - Funding Information: This study was supported by a grant from Korea University and also supported by Kmep (T28021) to Jong-Soon Choi.

PY - 2008/11/29

Y1 - 2008/11/29

N2 - Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

AB - Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

KW - ACC

KW - AMPK

KW - CaMKK

KW - Calcium

KW - Oxytocin

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SN - 0167-0115

VL - 151

SP - 71

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JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 1-3

ER -

Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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