Abstract
We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.
Original language | English |
---|---|
Pages (from-to) | 1207-1211 |
Number of pages | 5 |
Journal | European Food Research and Technology |
Volume | 235 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2012 Dec |
Bibliographical note
Funding Information:Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.
Keywords
- Melanogenesis
- Microphthalmia-associated transcription factor
- Tyrosinase
- cAMP-responsive element-binding protein
- p-Coumaric acid
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Biochemistry
- General Chemistry
- Industrial and Manufacturing Engineering