p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Hee jin Jun, Ji Hae Lee, Bo Ram Cho, Woo Duck Seo, Dong Woo Kim, Kang Jin Cho, Sung Joon Lee

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

Original languageEnglish
Pages (from-to)1207-1211
Number of pages5
JournalEuropean Food Research and Technology
Issue number6
Publication statusPublished - 2012 Dec


  • Melanogenesis
  • Microphthalmia-associated transcription factor
  • Tyrosinase
  • cAMP-responsive element-binding protein
  • p-Coumaric acid

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Biochemistry
  • Chemistry(all)
  • Industrial and Manufacturing Engineering


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