p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Hee jin Jun; Ji Hae Lee; Bo Ram Cho; Woo Duck Seo; Dong Woo Kim; Kang Jin Cho; Sung Joon Lee

doi:10.1007/s00217-012-1830-8

p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Hee jin Jun, Ji Hae Lee, Bo Ram Cho, Woo Duck Seo, Dong Woo Kim, Kang Jin Cho, Sung Joon Lee

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

Original language	English
Pages (from-to)	1207-1211
Number of pages	5
Journal	European Food Research and Technology
Volume	235
Issue number	6
DOIs	https://doi.org/10.1007/s00217-012-1830-8
Publication status	Published - 2012 Dec

Bibliographical note

Funding Information:
Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.

Keywords

Melanogenesis
Microphthalmia-associated transcription factor
Tyrosinase
cAMP-responsive element-binding protein
p-Coumaric acid

ASJC Scopus subject areas

Biotechnology
Food Science
Biochemistry
General Chemistry
Industrial and Manufacturing Engineering

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10.1007/s00217-012-1830-8

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title = "p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis",

abstract = "We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.",

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author = "Jun, {Hee jin} and Lee, {Ji Hae} and Cho, {Bo Ram} and Seo, {Woo Duck} and Kim, {Dong Woo} and Cho, {Kang Jin} and Lee, {Sung Joon}",

note = "Funding Information: Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.",

year = "2012",

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doi = "10.1007/s00217-012-1830-8",

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AU - Cho, Bo Ram

AU - Seo, Woo Duck

AU - Kim, Dong Woo

AU - Cho, Kang Jin

AU - Lee, Sung Joon

N1 - Funding Information: Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.

PY - 2012/12

Y1 - 2012/12

N2 - We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

AB - We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

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KW - Microphthalmia-associated transcription factor

KW - Tyrosinase

KW - cAMP-responsive element-binding protein

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p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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