p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Hee jin Jun; Ji Hae Lee; Bo Ram Cho; Woo Duck Seo; Dong Woo Kim; Kang Jin Cho; Sung Joon Lee

doi:10.1007/s00217-012-1830-8

p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Hee jin Jun
, Ji Hae Lee
, Bo Ram Cho
, Woo Duck Seo
, Dong Woo Kim
, Kang Jin Cho
, Sung Joon Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

Original language	English
Pages (from-to)	1207-1211
Number of pages	5
Journal	European Food Research and Technology
Volume	235
Issue number	6
DOIs	https://doi.org/10.1007/s00217-012-1830-8
Publication status	Published - 2012 Dec

Bibliographical note

Funding Information:
Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.

Keywords

Melanogenesis
Microphthalmia-associated transcription factor
Tyrosinase
cAMP-responsive element-binding protein
p-Coumaric acid

ASJC Scopus subject areas

Biotechnology
Food Science
Biochemistry
General Chemistry
Industrial and Manufacturing Engineering

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10.1007/s00217-012-1830-8

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title = "p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis",

abstract = "We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 \% vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 \% compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 \% vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 \% vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.",

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author = "Jun, \{Hee jin\} and Lee, \{Ji Hae\} and Cho, \{Bo Ram\} and Seo, \{Woo Duck\} and Kim, \{Dong Woo\} and Cho, \{Kang Jin\} and Lee, \{Sung Joon\}",

note = "Funding Information: Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science \& Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.",

year = "2012",

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doi = "10.1007/s00217-012-1830-8",

language = "English",

volume = "235",

pages = "1207--1211",

journal = "European Food Research and Technology",

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AU - Jun, Hee jin

AU - Lee, Ji Hae

AU - Cho, Bo Ram

AU - Seo, Woo Duck

AU - Kim, Dong Woo

AU - Cho, Kang Jin

AU - Lee, Sung Joon

N1 - Funding Information: Acknowledgments This work was conducted with support from the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480, No. 201202010305160010400, and No. 201203013026150010400) Rural Development Administration, Republic of Korea.

PY - 2012/12

Y1 - 2012/12

N2 - We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

AB - We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

KW - Melanogenesis

KW - Microphthalmia-associated transcription factor

KW - Tyrosinase

KW - cAMP-responsive element-binding protein

KW - p-Coumaric acid

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DO - 10.1007/s00217-012-1830-8

M3 - Article

AN - SCOPUS:84869498242

SN - 1438-2377

VL - 235

SP - 1207

EP - 1211

JO - European Food Research and Technology

JF - European Food Research and Technology

IS - 6

ER -

p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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