Abstract
P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.
Original language | English |
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Pages (from-to) | 1169-1175 |
Number of pages | 7 |
Journal | Biotechnology letters |
Volume | 30 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2008 Jul |
Keywords
- Carcinoma cells
- Dimethyl sulphoxide
- Embryoid body
- Endothelial cell differentiation
- P19 embryonal carcinoma cells
- Vascular endothelial growth factor
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology