P19 Embryonal carcinoma cells: A new model for the study of endothelial cell differentiation

Seung Cheol Choi, Ji Hyun Choi, Wan Joo Shim, Do Sun Lim

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.

    Original languageEnglish
    Pages (from-to)1169-1175
    Number of pages7
    JournalBiotechnology letters
    Volume30
    Issue number7
    DOIs
    Publication statusPublished - 2008 Jul

    Keywords

    • Carcinoma cells
    • Dimethyl sulphoxide
    • Embryoid body
    • Endothelial cell differentiation
    • P19 embryonal carcinoma cells
    • Vascular endothelial growth factor

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Fingerprint

    Dive into the research topics of 'P19 Embryonal carcinoma cells: A new model for the study of endothelial cell differentiation'. Together they form a unique fingerprint.

    Cite this