P19 Embryonal carcinoma cells: A new model for the study of endothelial cell differentiation

Seung Cheol Choi; Ji Hyun Choi; Wan Joo Shim; Do Sun Lim

doi:10.1007/s10529-008-9677-6

P19 Embryonal carcinoma cells: A new model for the study of endothelial cell differentiation

Seung Cheol Choi, Ji Hyun Choi, Wan Joo Shim, Do Sun Lim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.

Original language	English
Pages (from-to)	1169-1175
Number of pages	7
Journal	Biotechnology letters
Volume	30
Issue number	7
DOIs	https://doi.org/10.1007/s10529-008-9677-6
Publication status	Published - 2008 Jul

Keywords

Carcinoma cells
Dimethyl sulphoxide
Embryoid body
Endothelial cell differentiation
P19 embryonal carcinoma cells
Vascular endothelial growth factor

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "P19 Embryonal carcinoma cells: A new model for the study of endothelial cell differentiation",

abstract = "P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.",

keywords = "Carcinoma cells, Dimethyl sulphoxide, Embryoid body, Endothelial cell differentiation, P19 embryonal carcinoma cells, Vascular endothelial growth factor",

author = "Choi, {Seung Cheol} and Choi, {Ji Hyun} and Shim, {Wan Joo} and Lim, {Do Sun}",

year = "2008",

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language = "English",

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T1 - P19 Embryonal carcinoma cells

T2 - A new model for the study of endothelial cell differentiation

AU - Choi, Seung Cheol

AU - Choi, Ji Hyun

AU - Shim, Wan Joo

AU - Lim, Do Sun

PY - 2008/7

Y1 - 2008/7

N2 - P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.

AB - P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.

KW - Carcinoma cells

KW - Dimethyl sulphoxide

KW - Embryoid body

KW - Endothelial cell differentiation

KW - P19 embryonal carcinoma cells

KW - Vascular endothelial growth factor

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EP - 1175

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P19 Embryonal carcinoma cells: A new model for the study of endothelial cell differentiation

Abstract

Keywords

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