Paper-based preconcentration and isolation of microvesicles and exosomes

Dohwan Lee, Kyung Wook Wee, Hyerin Kim, Sung Il Han, Seungmin Kwak, Dae Sung Yoon, Jeong Hoon Lee

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Microvesicles and exosomes are small membranous vesicles released to the extracellular environment and circulated throughout the body. Because they contain various parental cell-derived biomolecules such as DNA, mRNA, miRNA, proteins, and lipids, their enrichment and isolation are critical steps for their exploitation as potential biomarkers for clinical applications. However, conventional isolation methods (e.g., ultracentrifugation) cause significant loss and damage to microvesicles and exosomes. These methods also require multiple repetitive steps of ultracentrifugation, loading, and wasting of reagents. This article describes a detailed method to fabricate an origami-paper-based device (Exo-PAD) designed for the effective enrichment and isolation of microvesicles and exosomes in a simple manner. The unique design of the Exo-PAD, consisting of accordion-like multifolded layers with convergent sample areas, is integrated with the ion concentration polarization technique, thereby enabling fivefold enrichment of the microvesicles and exosomes on specific layers. In addition, the enriched microvesicles and exosomes are isolated by simply unfolding the Exo-PAD.

    Original languageEnglish
    Article numbere61292
    JournalJournal of Visualized Experiments
    Volume2020
    Issue number158
    DOIs
    Publication statusPublished - 2020 Apr

    Bibliographical note

    Publisher Copyright:
    © 2020 Journal of Visualized Experiments.

    Keywords

    • Biochemistry
    • Exosome
    • Ion concentration polarization
    • Issue 158
    • Microvesicle
    • Paper-based microfluidics
    • Sample preconcentration
    • Sample preparation

    ASJC Scopus subject areas

    • General Neuroscience
    • General Chemical Engineering
    • General Biochemistry,Genetics and Molecular Biology
    • General Immunology and Microbiology

    Fingerprint

    Dive into the research topics of 'Paper-based preconcentration and isolation of microvesicles and exosomes'. Together they form a unique fingerprint.

    Cite this