Pathological Characteristics of Monosodium Iodoacetate-Induced Osteoarthritis in Rats

Minji Kwon, Dongyeon Nam, Junesun Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


BACKGROUND:: This study aimed to identify pain-related behavior and pathological characteristics of the knee joint in rats with monosodium iodoacetate (MIA)-induced osteoarthritis (OA). METHODS:: Knee joint inflammation was induced by intra-articular injection of MIA (4 mg/50 µL, n = 14) in 6-week-old male rats. Knee joint diameter, weight-bearing percentage on the hind limb during walking, the knee bending score, and paw withdrawal to mechanical stimuli were measured to evaluate edema and pain-related behavior for 28 d after MIA injection. Histological changes in the knee joints were evaluated using safranin O fast green staining on days 1, 3, 5, 7, 14, and 28 after OA induction (n = 3, respectively). Changes in bone structure and bone mineral density (BMD) were examined 14 and 28 d after OA (n = 3, respectively) using micro-computed tomography (CT). RESULTS:: The knee joint diameter and knee bending scores of the ipsilateral joint significantly increased 1 d after MIA injection, and the increased knee joint diameter and knee bending score persisted for 28 d. Weight-bearing during walking and paw withdrawal threshold (PWT) decreased from 1 and 5 d, respectively, and were maintained up to 28 d after MIA. Cartilage destruction started on day 1, and Mankin scores for bone destruction significantly increased for 14 d, as shown by micro-CT imaging. CONCLUSION:: The present study demonstrated that histopathological structural changes in the knee joint due to inflammation started soon after MIA injection, which induced OA pain from inflammation-related acute pain to spontaneous and evoked associated chronic pain.

Original languageEnglish
Pages (from-to)435-446
Number of pages12
JournalTissue Engineering and Regenerative Medicine
Issue number3
Publication statusPublished - 2023 Jun

Bibliographical note

Funding Information:
This work was supported by grants from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI21C0572), the Basic Science Research Program (2019R1I1A2A01060115) funded by the Ministry of Science, and the Korea University College of Health Science (K2120571).

Publisher Copyright:
© 2023, Korean Tissue Engineering and Regenerative Medicine Society.


  • Arthritic pain
  • Articular cartilage
  • Histopathology
  • Monosodium iodoacetate model
  • Osteoarthritis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering


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