TY - JOUR
T1 - Pause-and-stop
T2 - The effects of osmotic stress on cell proliferation during early leaf development in Arabidopsis and a role for ethylene signaling in cell cycle arrest
AU - Skirycz, Aleksandra
AU - Claeys, Hannes
AU - de Bodt, Stefanie
AU - Oikawa, Akira
AU - Shinoda, Shoko
AU - Andriankaja, Megan
AU - Maleux, Katrien
AU - Eloy, Nubia Barbosa
AU - Coppens, Frederik
AU - Yoo, Sang Dong
AU - Saito, Kazuki
AU - Inzé, Dirk
PY - 2011/5
Y1 - 2011/5
N2 - Despite its relevance for agricultural production, environmental stress-induced growth inhibition, which is responsible for-significant yield reductions, is only poorly understood. Here, we investigated the molecular mechanisms underlying cell-cycle inhibition in young proliferating leaves of the model plant Arabidopsis thaliana when subjected to mild osmotic stress. A detailed cellular analysis demonstrated that as soon as osmotic stress is sensed, cell cycle progression rapidly arrests,-but cells are kept in a latent ambivalent state allowing a quick recovery (pause). Remarkably, cell cycle arrest coincides with-an increase in 1-aminocyclopropane-1-carboxylate levels and the activation of ethylene signaling. Our work showed that-ethylene acts on cell cycle progression via inhibition of cyclin-dependent kinase A activity independently of EIN3 transcriptional-control. When the stress persists, cells exit the mitotic cell cycle and initiate the differentiation process (stop).-This stop is reflected by early endore duplication onset, in a process independent of ethylene. Nonetheless, the potential to-partially recover the decreased cell numbers remains due to the activity of meristemoids. Together, these data present a-conceptual framework to understand how environmental stress reduces plant growth.
AB - Despite its relevance for agricultural production, environmental stress-induced growth inhibition, which is responsible for-significant yield reductions, is only poorly understood. Here, we investigated the molecular mechanisms underlying cell-cycle inhibition in young proliferating leaves of the model plant Arabidopsis thaliana when subjected to mild osmotic stress. A detailed cellular analysis demonstrated that as soon as osmotic stress is sensed, cell cycle progression rapidly arrests,-but cells are kept in a latent ambivalent state allowing a quick recovery (pause). Remarkably, cell cycle arrest coincides with-an increase in 1-aminocyclopropane-1-carboxylate levels and the activation of ethylene signaling. Our work showed that-ethylene acts on cell cycle progression via inhibition of cyclin-dependent kinase A activity independently of EIN3 transcriptional-control. When the stress persists, cells exit the mitotic cell cycle and initiate the differentiation process (stop).-This stop is reflected by early endore duplication onset, in a process independent of ethylene. Nonetheless, the potential to-partially recover the decreased cell numbers remains due to the activity of meristemoids. Together, these data present a-conceptual framework to understand how environmental stress reduces plant growth.
UR - http://www.scopus.com/inward/record.url?scp=79959839056&partnerID=8YFLogxK
U2 - 10.1105/tpc.111.084160
DO - 10.1105/tpc.111.084160
M3 - Article
C2 - 21558544
AN - SCOPUS:79959839056
SN - 1040-4651
VL - 23
SP - 1876
EP - 1888
JO - Plant Cell
JF - Plant Cell
IS - 5
ER -