TY - JOUR
T1 - PCR-like performance of rapid test with permselective tunable nanotrap
AU - Park, Seong Jun
AU - Lee, Seungmin
AU - Lee, Dongtak
AU - Lee, Na Eun
AU - Park, Jeong Soo
AU - Hong, Ji Hye
AU - Jang, Jae Won
AU - Kim, Hyunji
AU - Roh, Seokbeom
AU - Lee, Gyudo
AU - Lee, Dongho
AU - Cho, Sung Yeon
AU - Park, Chulmin
AU - Lee, Dong Gun
AU - Lee, Raeseok
AU - Nho, Dukhee
AU - Yoon, Dae Sung
AU - Yoo, Yong Kyoung
AU - Lee, Jeong Hoon
N1 - Publisher Copyright:
© 2023. The Author(s).
PY - 2023/3/18
Y1 - 2023/3/18
N2 - Highly sensitive rapid testing for COVID-19 is essential for minimizing virus transmission, especially before the onset of symptoms and in asymptomatic cases. Here, we report bioengineered enrichment tools for lateral flow assays (LFAs) with enhanced sensitivity and specificity (BEETLES2), achieving enrichment of SARS-CoV-2 viruses, nucleocapsid (N) proteins and immunoglobulin G (IgG) with 3-minute operation. The limit of detection is improved up to 20-fold. We apply this method to clinical samples, including 83% with either intermediate (35%) or low viral loads (48%), collected from 62 individuals (n = 42 for positive and n = 20 for healthy controls). We observe diagnostic sensitivity, specificity, and accuracy of 88.1%, 100%, and 91.9%, respectively, compared with commercial LFAs alone achieving 14.29%, 100%, and 41.94%, respectively. BEETLES2, with permselectivity and tunability, can enrich the SARS-CoV-2 virus, N proteins, and IgG in the nasopharyngeal/oropharyngeal swab, saliva, and blood serum, enabling reliable and sensitive point-of-care testing, facilitating fast early diagnosis.
AB - Highly sensitive rapid testing for COVID-19 is essential for minimizing virus transmission, especially before the onset of symptoms and in asymptomatic cases. Here, we report bioengineered enrichment tools for lateral flow assays (LFAs) with enhanced sensitivity and specificity (BEETLES2), achieving enrichment of SARS-CoV-2 viruses, nucleocapsid (N) proteins and immunoglobulin G (IgG) with 3-minute operation. The limit of detection is improved up to 20-fold. We apply this method to clinical samples, including 83% with either intermediate (35%) or low viral loads (48%), collected from 62 individuals (n = 42 for positive and n = 20 for healthy controls). We observe diagnostic sensitivity, specificity, and accuracy of 88.1%, 100%, and 91.9%, respectively, compared with commercial LFAs alone achieving 14.29%, 100%, and 41.94%, respectively. BEETLES2, with permselectivity and tunability, can enrich the SARS-CoV-2 virus, N proteins, and IgG in the nasopharyngeal/oropharyngeal swab, saliva, and blood serum, enabling reliable and sensitive point-of-care testing, facilitating fast early diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=85150666486&partnerID=8YFLogxK
U2 - 10.1038/s41467-023-37018-6
DO - 10.1038/s41467-023-37018-6
M3 - Article
C2 - 36934093
AN - SCOPUS:85150666486
SN - 2041-1723
VL - 14
SP - 1520
JO - Nature Communications
JF - Nature Communications
IS - 1
ER -