PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

Jiwon Mo, Sukwoo Choi, Poong Gi Ahn, Woong Sun, Hyun Woo Lee, Hyun Kim

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.

Original languageEnglish
Pages (from-to)250-255
Number of pages6
JournalBiochemical and biophysical research communications
Issue number2
Publication statusPublished - 2012 Jun 1

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) Grants 2011-0019227 , 2011-0019229 , and 2010K000830 . We thank Dr. Eunjoon Kim (Korea Advanced Institute Science and Technology) for the kind gift of Arf6 and EFA6A antibodies.


  • Arf6
  • Dendritic development
  • EFA6A
  • F-actin
  • MAP2
  • Tamalin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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