PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

Jiwon Mo; Sukwoo Choi; Poong Gi Ahn; Woong Sun; Hyun Woo Lee; Hyun Kim

doi:10.1016/j.bbrc.2012.04.136

PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

Jiwon Mo, Sukwoo Choi, Poong Gi Ahn, Woong Sun, Hyun Woo Lee, Hyun Kim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.

Original language	English
Pages (from-to)	250-255
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	422
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2012.04.136
Publication status	Published - 2012 Jun 1

Keywords

Arf6
Dendritic development
EFA6A
F-actin
MAP2
Tamalin

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2012.04.136

Cite this

@article{2abbc0c721e44b77b18fd44824d99a6e,

title = "PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron",

abstract = "Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.",

keywords = "Arf6, Dendritic development, EFA6A, F-actin, MAP2, Tamalin",

author = "Jiwon Mo and Sukwoo Choi and Ahn, {Poong Gi} and Woong Sun and Lee, {Hyun Woo} and Hyun Kim",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) Grants 2011-0019227 , 2011-0019229 , and 2010K000830 . We thank Dr. Eunjoon Kim (Korea Advanced Institute Science and Technology) for the kind gift of Arf6 and EFA6A antibodies.",

year = "2012",

month = jun,

day = "1",

doi = "10.1016/j.bbrc.2012.04.136",

language = "English",

volume = "422",

pages = "250--255",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

AU - Mo, Jiwon

AU - Choi, Sukwoo

AU - Ahn, Poong Gi

AU - Sun, Woong

AU - Lee, Hyun Woo

AU - Kim, Hyun

N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) Grants 2011-0019227 , 2011-0019229 , and 2010K000830 . We thank Dr. Eunjoon Kim (Korea Advanced Institute Science and Technology) for the kind gift of Arf6 and EFA6A antibodies.

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.

AB - Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.

KW - Arf6

KW - Dendritic development

KW - EFA6A

KW - F-actin

KW - MAP2

KW - Tamalin

UR - http://www.scopus.com/inward/record.url?scp=84861665488&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2012.04.136

DO - 10.1016/j.bbrc.2012.04.136

M3 - Article

C2 - 22569042

AN - SCOPUS:84861665488

SN - 0006-291X

VL - 422

SP - 250

EP - 255

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this