Peli3 ablation ameliorates acetaminophen-induced liver injury through inhibition of GSK3β phosphorylation and mitochondrial translocation

Jaewon Lee, Jihoon Ha, Jun Hyeong Kim, Dongyeob Seo, Minbeom Kim, Yerin Lee, Seong Shil Park, Dahee Choi, Jin Seok Park, Young Jae Lee, Siyoung Yang, Kyung Min Yang, Su Myung Jung, Suntaek Hong, Seung Hoi Koo, Yong Soo Bae, Seong Jin Kim, Seok Hee Park

Research output: Contribution to journalArticlepeer-review

Abstract

The signaling pathways governing acetaminophen (APAP)-induced liver injury have been extensively studied. However, little is known about the ubiquitin-modifying enzymes needed for the regulation of APAP-induced liver injury. Here, we examined whether the Pellino3 protein, which has E3 ligase activity, is needed for APAP-induced liver injury and subsequently explored its molecular mechanism. Whole-body Peli3 −/− knockout (KO) and adenovirus-mediated Peli3 knockdown (KD) mice showed reduced levels of centrilobular cell death, infiltration of immune cells, and biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), upon APAP treatment compared to wild-type (WT) mice. Peli3 deficiency in primary hepatocytes decreased mitochondrial and lysosomal damage and reduced the mitochondrial reactive oxygen species (ROS) levels. In addition, the levels of phosphorylation at serine 9 in the cytoplasm and mitochondrial translocation of GSK3β were decreased in primary hepatocytes obtained from Peli3 −/− KO mice, and these reductions were accompanied by decreases in JNK phosphorylation and mitochondrial translocation. Pellino3 bound more strongly to GSK3β compared with JNK1 and JNK2 and induced the lysine 63 (K63)-mediated polyubiquitination of GSK3β. In rescue experiments, the ectopic expression of wild-type Pellino3 in Peli3 −/− KO hepatocytes restored the mitochondrial translocation of GSK3β, but this restoration was not obtained with expression of a catalytically inactive mutant of Pellino3. These findings are the first to suggest a mechanistic link between Pellino3 and APAP-induced liver injury through the modulation of GSK3β polyubiquitination.

Original languageEnglish
Pages (from-to)1218-1231
Number of pages14
JournalExperimental and Molecular Medicine
Volume55
Issue number6
DOIs
Publication statusPublished - 2023 Jun

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation grant of Korea (SRC 2017R1A5A1014560) funded by the Ministry of Science and ICT and in part by a grant (2020R1A2C3009643 to S.H.P.) from the National Research Foundation of Korea.

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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