Pendimethalin induces apoptosis in testicular cells via hampering ER-mitochondrial function and autophagy

Jiyeon Ham; Whasun Lim; Gwonhwa Song

doi:10.1016/j.envpol.2021.116835

Pendimethalin induces apoptosis in testicular cells via hampering ER-mitochondrial function and autophagy

Jiyeon Ham, Whasun Lim, Gwonhwa Song

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Pendimethalin (PDM) is a dinitroaniline crop pesticide that is extensively utilized worldwide. However, the reproductive toxicity and cellular mechanisms of PDM have not been identified. Therefore, we elucidated the adverse effects of PDM on the reproductive system using mouse testicular Leydig and Sertoli cells (TM3 and TM4 cells, respectively). Our results demonstrated that PDM suppressed the viability and proliferation of TM3 and TM4 cells. Additionally, PDM induced cytosolic calcium upregulation and permeabilization of mitochondrial membrane potential in both TM3 and TM4 cells. We also verified that PDM activates the endoplasmic reticulum (ER) stress pathway and autophagy. Furthermore, we confirmed that activation of ER stress and autophagy were blocked by 2-aminoethoxydiphenyl borate (2-APB) treatment. Finally, we confirmed PDM-induced cell cycle arrest and apoptosis in TM3 and TM4 cells. Thus, we first demonstrated that PDM impedes the survival of testis cells, and further, their function.

Original language	English
Article number	116835
Journal	Environmental Pollution
Volume	278
DOIs	https://doi.org/10.1016/j.envpol.2021.116835
Publication status	Published - 2021 Jun 1

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (MSIT) (grant number: 2018R1C1B6009048 ) and suppported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Minisry of Education (grant number: 2020R1A6A3A13074214 ).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

Autophagy
ER stress
Herbicides
Inositol 1,4,5-triphosphate receptors
Pendimethalin

ASJC Scopus subject areas

Toxicology
Pollution
Health, Toxicology and Mutagenesis

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title = "Pendimethalin induces apoptosis in testicular cells via hampering ER-mitochondrial function and autophagy",

abstract = "Pendimethalin (PDM) is a dinitroaniline crop pesticide that is extensively utilized worldwide. However, the reproductive toxicity and cellular mechanisms of PDM have not been identified. Therefore, we elucidated the adverse effects of PDM on the reproductive system using mouse testicular Leydig and Sertoli cells (TM3 and TM4 cells, respectively). Our results demonstrated that PDM suppressed the viability and proliferation of TM3 and TM4 cells. Additionally, PDM induced cytosolic calcium upregulation and permeabilization of mitochondrial membrane potential in both TM3 and TM4 cells. We also verified that PDM activates the endoplasmic reticulum (ER) stress pathway and autophagy. Furthermore, we confirmed that activation of ER stress and autophagy were blocked by 2-aminoethoxydiphenyl borate (2-APB) treatment. Finally, we confirmed PDM-induced cell cycle arrest and apoptosis in TM3 and TM4 cells. Thus, we first demonstrated that PDM impedes the survival of testis cells, and further, their function.",

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author = "Jiyeon Ham and Whasun Lim and Gwonhwa Song",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (MSIT) (grant number: 2018R1C1B6009048 ) and suppported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Minisry of Education (grant number: 2020R1A6A3A13074214 ). Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

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doi = "10.1016/j.envpol.2021.116835",

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AU - Lim, Whasun

AU - Song, Gwonhwa

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (MSIT) (grant number: 2018R1C1B6009048 ) and suppported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Minisry of Education (grant number: 2020R1A6A3A13074214 ). Publisher Copyright: © 2021 Elsevier Ltd

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N2 - Pendimethalin (PDM) is a dinitroaniline crop pesticide that is extensively utilized worldwide. However, the reproductive toxicity and cellular mechanisms of PDM have not been identified. Therefore, we elucidated the adverse effects of PDM on the reproductive system using mouse testicular Leydig and Sertoli cells (TM3 and TM4 cells, respectively). Our results demonstrated that PDM suppressed the viability and proliferation of TM3 and TM4 cells. Additionally, PDM induced cytosolic calcium upregulation and permeabilization of mitochondrial membrane potential in both TM3 and TM4 cells. We also verified that PDM activates the endoplasmic reticulum (ER) stress pathway and autophagy. Furthermore, we confirmed that activation of ER stress and autophagy were blocked by 2-aminoethoxydiphenyl borate (2-APB) treatment. Finally, we confirmed PDM-induced cell cycle arrest and apoptosis in TM3 and TM4 cells. Thus, we first demonstrated that PDM impedes the survival of testis cells, and further, their function.

AB - Pendimethalin (PDM) is a dinitroaniline crop pesticide that is extensively utilized worldwide. However, the reproductive toxicity and cellular mechanisms of PDM have not been identified. Therefore, we elucidated the adverse effects of PDM on the reproductive system using mouse testicular Leydig and Sertoli cells (TM3 and TM4 cells, respectively). Our results demonstrated that PDM suppressed the viability and proliferation of TM3 and TM4 cells. Additionally, PDM induced cytosolic calcium upregulation and permeabilization of mitochondrial membrane potential in both TM3 and TM4 cells. We also verified that PDM activates the endoplasmic reticulum (ER) stress pathway and autophagy. Furthermore, we confirmed that activation of ER stress and autophagy were blocked by 2-aminoethoxydiphenyl borate (2-APB) treatment. Finally, we confirmed PDM-induced cell cycle arrest and apoptosis in TM3 and TM4 cells. Thus, we first demonstrated that PDM impedes the survival of testis cells, and further, their function.

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Pendimethalin induces apoptosis in testicular cells via hampering ER-mitochondrial function and autophagy

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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