Peptides from Anchovy Sauce Induce Apoptosis in a Human Lymphoma Cell (U937) through the Increase of Caspase-3 and -8 Activities

Young Gon Lee; Ji Yeon Kim; Ki Won Lee; Kyoung Heon Kim; Hyong Joo Lee

doi:10.1196/annals.1299.073

Peptides from Anchovy Sauce Induce Apoptosis in a Human Lymphoma Cell (U937) through the Increase of Caspase-3 and -8 Activities

Young Gon Lee, Ji Yeon Kim, Ki Won Lee, Kyoung Heon Kim, Hyong Joo Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Various biological activities of peptides have been found. In this study, the induction of apoptosis in a human lymphoma cell line (U937) by peptide fraction separated from anchovy sauce was studied using biochemical and flow cytometric methods. After U937 was treated with a hydrophobic peptide fraction of the anchovy sauce, a sub-G1 peak, which represents apoptosis, was found in the cell cycle analysis. The apoptosis in the peptide-treated U937 cell was also confirmed by apoptotic DNA fragmentation. Furthermore, an increase of caspase-3 and -8 activities was observed. Our results suggest that the peptide fraction separated from the anchovy sauce may have cancer chemopreventive effects through the induction of apoptosis in cancer cells.

Original language	English
Pages (from-to)	399-404
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	1010
DOIs	https://doi.org/10.1196/annals.1299.073
Publication status	Published - 2003

Keywords

Apoptosis
Caspase
Fish sauce
Lymphoma cell line
Peptide

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1196/annals.1299.073

Cite this

@article{35d85135e086489484da784b24acc8d6,

title = "Peptides from Anchovy Sauce Induce Apoptosis in a Human Lymphoma Cell (U937) through the Increase of Caspase-3 and -8 Activities",

abstract = "Various biological activities of peptides have been found. In this study, the induction of apoptosis in a human lymphoma cell line (U937) by peptide fraction separated from anchovy sauce was studied using biochemical and flow cytometric methods. After U937 was treated with a hydrophobic peptide fraction of the anchovy sauce, a sub-G1 peak, which represents apoptosis, was found in the cell cycle analysis. The apoptosis in the peptide-treated U937 cell was also confirmed by apoptotic DNA fragmentation. Furthermore, an increase of caspase-3 and -8 activities was observed. Our results suggest that the peptide fraction separated from the anchovy sauce may have cancer chemopreventive effects through the induction of apoptosis in cancer cells.",

keywords = "Apoptosis, Caspase, Fish sauce, Lymphoma cell line, Peptide",

author = "Lee, {Young Gon} and Kim, {Ji Yeon} and Lee, {Ki Won} and Kim, {Kyoung Heon} and Lee, {Hyong Joo}",

year = "2003",

doi = "10.1196/annals.1299.073",

language = "English",

volume = "1010",

pages = "399--404",

journal = "Annals of The Lyceum of Natural History of New York",

issn = "0890-6564",

publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Peptides from Anchovy Sauce Induce Apoptosis in a Human Lymphoma Cell (U937) through the Increase of Caspase-3 and -8 Activities

AU - Lee, Young Gon

AU - Kim, Ji Yeon

AU - Lee, Ki Won

AU - Kim, Kyoung Heon

AU - Lee, Hyong Joo

PY - 2003

Y1 - 2003

N2 - Various biological activities of peptides have been found. In this study, the induction of apoptosis in a human lymphoma cell line (U937) by peptide fraction separated from anchovy sauce was studied using biochemical and flow cytometric methods. After U937 was treated with a hydrophobic peptide fraction of the anchovy sauce, a sub-G1 peak, which represents apoptosis, was found in the cell cycle analysis. The apoptosis in the peptide-treated U937 cell was also confirmed by apoptotic DNA fragmentation. Furthermore, an increase of caspase-3 and -8 activities was observed. Our results suggest that the peptide fraction separated from the anchovy sauce may have cancer chemopreventive effects through the induction of apoptosis in cancer cells.

AB - Various biological activities of peptides have been found. In this study, the induction of apoptosis in a human lymphoma cell line (U937) by peptide fraction separated from anchovy sauce was studied using biochemical and flow cytometric methods. After U937 was treated with a hydrophobic peptide fraction of the anchovy sauce, a sub-G1 peak, which represents apoptosis, was found in the cell cycle analysis. The apoptosis in the peptide-treated U937 cell was also confirmed by apoptotic DNA fragmentation. Furthermore, an increase of caspase-3 and -8 activities was observed. Our results suggest that the peptide fraction separated from the anchovy sauce may have cancer chemopreventive effects through the induction of apoptosis in cancer cells.

KW - Apoptosis

KW - Caspase

KW - Fish sauce

KW - Lymphoma cell line

KW - Peptide

UR - http://www.scopus.com/inward/record.url?scp=1342305482&partnerID=8YFLogxK

U2 - 10.1196/annals.1299.073

DO - 10.1196/annals.1299.073

M3 - Article

C2 - 15033760

AN - SCOPUS:1342305482

SN - 0890-6564

VL - 1010

SP - 399

EP - 404

JO - Annals of The Lyceum of Natural History of New York

JF - Annals of The Lyceum of Natural History of New York

ER -

Peptides from Anchovy Sauce Induce Apoptosis in a Human Lymphoma Cell (U937) through the Increase of Caspase-3 and -8 Activities

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this