Performance Evaluation of Alere i Influenza A&B in Detecting Influenza Viruses A and B

Ha Nui Kim, Woong Sik Jang, Jeonghun Nam, Do Cic Mihn, Chae Seung Lim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Objective. Alere i Influenza A&B is an isothermal nucleic acid amplification-based integrated system used for detecting and differentiating between influenza virus A and influenza virus B. We evaluated the clinical performances of Alere i Influenza A&B compared to that of real-time PCR, multiplex real-time PCR, and two rapid influenza diagnostic kits. Methods. Nasopharyngeal aspiration specimens (n=315) from patients with signs of acute respiratory infection were collected between 2015 and 2016. Samples were tested using real-time PCR, the multiplex RT-PCR Anyplex II RV16 Detection kit, Alere i Influenza A&B, BD Veritor™ System Flu A+B, and the Sofia Influenza A+B Fluorescence Immunoassay. Positive influenza specimens detected by the Anyplex II RV16 Detection kit were tested by real-time PCR. Results. Compared to that of multiplex RT-PCR (influenza A, n=88; influenza B, n=82; influenza-negative, n=145), the sensitivities of Alere i, Sofia, and Veritor for influenza A were 97.7%, 72.7%, and 71.6%, respectively, whereas for influenza B, the sensitivities were 96.3%, 80.4%, and 75.6%, respectively. The specificity of Alere i, Sofia, and Veritor was 100.0%. Conclusions.The clinical performance of Alere i Influenza A&B is satisfactory, with the advantage of a significantly shorter test time than other molecular assays. It is suitable for point-of-care testing and rapid influenza diagnostic tests because of its high sensitivity and specificity.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalAnnals of Clinical and Laboratory Science
Issue number1
Publication statusPublished - 2021 Jan

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) with the grant NRF-2016R1A5A1010148, a government-wide R&D fund for infectious disease research (HG18C0012), and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HR20C0021).

Publisher Copyright:
© 2021 by the Association of Clinical Scientists, Inc.


  • Alere i Influenza A&B
  • RIDT
  • Real-time PCR
  • influenza

ASJC Scopus subject areas

  • General Medicine


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