Performance evaluation of newly developed surrogate virus neutralization tests for detecting neutralizing antibodies against SARS-CoV-2

Oh Joo Kweon, Joon Yong Bae, Yong Kwan Lim, Yoojeong Choi, Sohyun Lee, Man Seong Park, In Bum Suh, Hana Kim, Young Sam Jee, Mi Kyung Lee

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1 Citation (Scopus)

Abstract

We evaluated newly developed surrogate virus neutralization tests (sVNT) for detecting neutralizing antibodies (NAbs) against the receptor binding domain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). VERI-Q SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (MiCo BioMed, Gyeonggi-do, Republic of Korea, hereafter, “eCoV-CN”) is an enzyme-linked immunosorbent assay-based sVNT, and VERI-Q SARS-CoV-2 Neutralizing Antibody Rapid Test Kit (MiCo BioMed, hereafter, “rCoV-RN”) is a point-of-care lateral-flow immunochromatography test with auto-scanner. A total of 411 serum samples were evaluated. Both evaluations used a 50% plaque reduction neutralization test (PRNT50) as the gold standard. Compared with PRNT50, the eCoV-CN showed 98.7% positive percent agreement (PPA), 96.8% negative percent agreement (NPA), 97.4% total percent agreement (TPA), with kappa values of 0.942. The rCoV-RN showed 98.7% PPA, 97.4% NPA, 97.8% TPA, and kappa values of 0.951, comparing to PRNT50. Neither assay indicated cross-reactivity for other pathogens, and the signal indexes were statistically significantly correlated to the PRNT50 titer. The two evaluated sVNTs show comparable performances to the PRNT50 with the advantages of technical simplicity, speed, and do not require cell culture facilities.

Original languageEnglish
Article number4961
JournalScientific reports
Volume13
Issue number1
DOIs
Publication statusPublished - 2023 Dec

Bibliographical note

Funding Information:
We thank MiCo BioMed Inc. for providing the VERI-Q SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit and VERI-Q SARS-CoV-2 Neutralizing Antibody Rapid Test Kit for this study. MiCo BioMed Inc. provided technical support and had no role in the study design, data collection, or interpretation. The human-derived materials were provided by Chung-Ang University Hospital Human Biobank.

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant by the Ministry of Science and ICT (MSIT), Republic of Korea (Grant No. 2020R1A5A1018052 and NRF-2022M3A9I2017241).

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

  • General

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