Permselective glucose sensing with GLUT1-rich cancer cell membranes

Insu Kim, Dohyung Kwon, Dongtak Lee, Gyudo Lee, Dae Sung Yoon

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from MDA-MB-231 cells and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-(−)-fructose, D-(+)-xylose, D-(+)-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35–7.35 mM of glucose), implying their high potential for practical use.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalBiosensors and Bioelectronics
Publication statusPublished - 2019 Jun 15

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MSIP) (No. NRF-2016R1A2B4010269, NRF-2017R1A6A3A11034311 and NRF-2018M3C1B7020722). This material is also supported by the Ministry of Trade, Industry and Energy under Industrial Technology Innovation Program (No.10079316). Dr. Gyudo Lee is thankful for the financial support by the Korea University Grant.

Publisher Copyright:
© 2019 Elsevier B.V.


  • Cancer cell membrane
  • Electrochemical analysis
  • Glucose biosensor
  • Glucose transporter-1
  • Permselectivity

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


Dive into the research topics of 'Permselective glucose sensing with GLUT1-rich cancer cell membranes'. Together they form a unique fingerprint.

Cite this