Persistent infection of a gammaherpesvirus in the central nervous system

Hye Ri Kang; Hye Jeong Cho; Sungbum Kim; In Ho Song; Tae Sup Lee; Seungmin Hwang; Ren Sun; Moon Jung Song

doi:10.1016/j.virol.2011.11.012

Persistent infection of a gammaherpesvirus in the central nervous system

Hye Ri Kang
, Hye Jeong Cho
, Sungbum Kim
, In Ho Song
, Tae Sup Lee
, Seungmin Hwang
, Ren Sun
, Moon Jung Song^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Human gammaherpesvirus infections of the central nervous system (CNS) have been linked to various neurological diseases. Murine gammaherpesvirus 68 (MHV-68), genetically related and biologically similar to human gammaherpesviruses, infects the CNS in laboratory mice. However, viral persistency of MHV-68 has not been studied following CNS infection. In this study, we undertook the noninvasive bioluminescence imaging of a recombinant MHV-68 expressing the firefly luciferase (M3FL) to monitor virus progression after CNS infection. The M3FL virus inoculated in the brain systemically spread to the abdominal area in bioluminescence imaging, which was further confirmed by detection of viral genome and transcripts. The disseminated wild-type virus established latency in the spleen. Moreover, the treatment of the infected mice with CsA induced reactivation of latent MHV-68 from the brain and the spleen. Our results suggest that MHV-68 may persist both inside and outside the CNS once it gains access to the CNS.

Original language	English
Pages (from-to)	23-29
Number of pages	7
Journal	Virology
Volume	423
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2011.11.012
Publication status	Published - 2012 Feb 5

Bibliographical note

Funding Information:
This work was supported by grants from the National R&D Program for Cancer Control, Ministry of Health, Welfare and Family Affairs, Republic of Korea ( 0920170 ) and from Mid-career Researcher Program through NRF grant funded by the MEST ( NRF-2010-0000484 ) to M.J.S.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bioluminescence imaging
Central nervous system
Gammaherpesvirus
Persistent infection

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2011.11.012

Cite this

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title = "Persistent infection of a gammaherpesvirus in the central nervous system",

abstract = "Human gammaherpesvirus infections of the central nervous system (CNS) have been linked to various neurological diseases. Murine gammaherpesvirus 68 (MHV-68), genetically related and biologically similar to human gammaherpesviruses, infects the CNS in laboratory mice. However, viral persistency of MHV-68 has not been studied following CNS infection. In this study, we undertook the noninvasive bioluminescence imaging of a recombinant MHV-68 expressing the firefly luciferase (M3FL) to monitor virus progression after CNS infection. The M3FL virus inoculated in the brain systemically spread to the abdominal area in bioluminescence imaging, which was further confirmed by detection of viral genome and transcripts. The disseminated wild-type virus established latency in the spleen. Moreover, the treatment of the infected mice with CsA induced reactivation of latent MHV-68 from the brain and the spleen. Our results suggest that MHV-68 may persist both inside and outside the CNS once it gains access to the CNS.",

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note = "Funding Information: This work was supported by grants from the National R\&D Program for Cancer Control, Ministry of Health, Welfare and Family Affairs, Republic of Korea ( 0920170 ) and from Mid-career Researcher Program through NRF grant funded by the MEST ( NRF-2010-0000484 ) to M.J.S.",

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doi = "10.1016/j.virol.2011.11.012",

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AU - Kang, Hye Ri

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AU - Kim, Sungbum

AU - Song, In Ho

AU - Lee, Tae Sup

AU - Hwang, Seungmin

AU - Sun, Ren

AU - Song, Moon Jung

N1 - Funding Information: This work was supported by grants from the National R&D Program for Cancer Control, Ministry of Health, Welfare and Family Affairs, Republic of Korea ( 0920170 ) and from Mid-career Researcher Program through NRF grant funded by the MEST ( NRF-2010-0000484 ) to M.J.S.

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N2 - Human gammaherpesvirus infections of the central nervous system (CNS) have been linked to various neurological diseases. Murine gammaherpesvirus 68 (MHV-68), genetically related and biologically similar to human gammaherpesviruses, infects the CNS in laboratory mice. However, viral persistency of MHV-68 has not been studied following CNS infection. In this study, we undertook the noninvasive bioluminescence imaging of a recombinant MHV-68 expressing the firefly luciferase (M3FL) to monitor virus progression after CNS infection. The M3FL virus inoculated in the brain systemically spread to the abdominal area in bioluminescence imaging, which was further confirmed by detection of viral genome and transcripts. The disseminated wild-type virus established latency in the spleen. Moreover, the treatment of the infected mice with CsA induced reactivation of latent MHV-68 from the brain and the spleen. Our results suggest that MHV-68 may persist both inside and outside the CNS once it gains access to the CNS.

AB - Human gammaherpesvirus infections of the central nervous system (CNS) have been linked to various neurological diseases. Murine gammaherpesvirus 68 (MHV-68), genetically related and biologically similar to human gammaherpesviruses, infects the CNS in laboratory mice. However, viral persistency of MHV-68 has not been studied following CNS infection. In this study, we undertook the noninvasive bioluminescence imaging of a recombinant MHV-68 expressing the firefly luciferase (M3FL) to monitor virus progression after CNS infection. The M3FL virus inoculated in the brain systemically spread to the abdominal area in bioluminescence imaging, which was further confirmed by detection of viral genome and transcripts. The disseminated wild-type virus established latency in the spleen. Moreover, the treatment of the infected mice with CsA induced reactivation of latent MHV-68 from the brain and the spleen. Our results suggest that MHV-68 may persist both inside and outside the CNS once it gains access to the CNS.

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KW - Persistent infection

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Persistent infection of a gammaherpesvirus in the central nervous system

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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