PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Chan Ho Jang; In Ae Lee; Young Ran Ha; Jinkyu Lim; Mi Kyung Sung; Sung Joon Lee; Jong Sang Kim

doi:10.1271/bbb.80079

PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Chan Ho Jang, In Ae Lee, Young Ran Ha, Jinkyu Lim, Mi Kyung Sung, Sung Joon Lee, Jong Sang Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

Original language	English
Pages (from-to)	1799-1808
Number of pages	10
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	7
DOIs	https://doi.org/10.1271/bbb.80079
Publication status	Published - 2008

Bibliographical note

Funding Information:
This study was supported by Korea Science & Engineering Foundation (R01-2005-000-10602-0) and a grant from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A05-0376-AD1104-06A2-00041B).

Keywords

Antioxidant
Biomarker
Oxidative stress
Phosphoglycerate kinase 1 (PGK1)

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells",

abstract = "Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.",

keywords = "Antioxidant, Biomarker, Oxidative stress, Phosphoglycerate kinase 1 (PGK1)",

author = "Jang, {Chan Ho} and Lee, {In Ae} and Ha, {Young Ran} and Jinkyu Lim and Sung, {Mi Kyung} and Lee, {Sung Joon} and Kim, {Jong Sang}",

note = "Funding Information: This study was supported by Korea Science & Engineering Foundation (R01-2005-000-10602-0) and a grant from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A05-0376-AD1104-06A2-00041B).",

year = "2008",

doi = "10.1271/bbb.80079",

language = "English",

volume = "72",

pages = "1799--1808",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

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TY - JOUR

T1 - PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

AU - Jang, Chan Ho

AU - Lee, In Ae

AU - Ha, Young Ran

AU - Lim, Jinkyu

AU - Sung, Mi Kyung

AU - Lee, Sung Joon

AU - Kim, Jong Sang

N1 - Funding Information: This study was supported by Korea Science & Engineering Foundation (R01-2005-000-10602-0) and a grant from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A05-0376-AD1104-06A2-00041B).

PY - 2008

Y1 - 2008

N2 - Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

AB - Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

KW - Antioxidant

KW - Biomarker

KW - Oxidative stress

KW - Phosphoglycerate kinase 1 (PGK1)

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U2 - 10.1271/bbb.80079

DO - 10.1271/bbb.80079

M3 - Article

C2 - 18603805

AN - SCOPUS:47949128698

SN - 0916-8451

VL - 72

SP - 1799

EP - 1808

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 7

ER -

PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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